Technology for preparing double-loaded or multi-loaded liposome through liposome fusion induction

Abstract

The invention belongs to the field of medicines and relates to a technology for preparing double-loaded or multi-loaded liposome through liposome fusion induction. The technology utilizes liposome fusion induction to prepare a double-loaded or multi-loaded liposome. The technology provides a feasible solution scheme for double-loaded or multi-loaded liposome preparation. The liposome realizes synergism of two or more drugs in vitro and vivo through colocalization of two or more drug structures and cell levels and provides a novel choice for clinical, safe and effective combined drug administration.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the method of inducing liposome fusion to prepare liposomes simultaneously carrying double or multiple drugs. Background technique [0002] It is a common phenomenon to use two or more therapeutic drugs in the treatment of diseases, especially for the treatment of tumors, it is often impossible to cure with one drug. If there is a synergistic therapeutic effect between two or more drugs, especially if the synergistic mechanism needs to be co-localized in the same target cell or target tissue, it is very difficult to co-load the two or more drugs in the same nanocarrier (referred to as co-carrier). It may be more helpful to improve drug efficacy than simple combined administration. This is mainly because co-carriers can deliver drugs closer to the optimal ratio and almost completely simultaneously to target cells or tissues. [0003] Liposome is one of the most important drug...

AI Technical Summary

Problems solved by technology

[0004] At present, in order to achieve the co-loading of two drugs, the commonly used method is to use the two-step "ion gradient method" (ie remoteloading). In order to form an ion gradient inside and outside the lipid bilayer membrane, it is often necessary to introduce ionic reagents (ie ionophores) Or metal ions such as Mn2+, Cu2+, Co2+, etc., these methods are often cumbersome and complex in composition

During the encapsulation process, the encapsulation efficiency of drug molecules is often reduced due to the simultaneous competition of different drugs for the binding sites of phospholipid molecules.

In addition, drugs that cannot be carried by the ion gradient method will challenge the method

Images