A dual-modality microscope imaging system and imaging method thereof

A micro-imaging, dual-mode technology, applied in the field of micro-imaging, can solve the problem of low phototoxicity

A micro-imaging, dual-mode technology, applied in the field of micro-imaging, can solve the problem of low phototoxicity

CN113702288BActive Publication Date: 2022-07-01PEKING UNIV
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  • A dual-modality microscope imaging system and imaging method thereof
  • A dual-modality microscope imaging system and imaging method thereof
  • A dual-modality microscope imaging system and imaging method thereof

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Embodiment Construction

[0037] Below in conjunction with the accompanying drawings, the present invention will be further described through specific embodiments.

[0038] like figure 1 and 2 As shown, the dual-mode microscopic imaging system combining two-photon fluorescence and optical diffraction tomography in this embodiment includes: an optical diffraction tomography subsystem, a two-photon fluorescence subsystem, a first dichroic mirror 115 , and a second objective lens 114 and control subsystem;

[0039] The optical diffraction tomography subsystem includes: a first light source 101, a first acousto-optic modulator 102, a first half-wave plate 103, a first polarization beam splitter 104, a first fiber coupler 105, a second fiber coupler 106, The first polarization-maintaining single-mode fiber 107, the second polarization-maintaining single-mode fiber 108, the scanning lens 111, the first dual-axis scanning galvanometer 110, the first objective lens 112, the first camera 130, the first tube l...

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Abstract

The invention discloses a dual-mode microscopic imaging system and an imaging method thereof. The invention combines the dual-mode microscopic imaging system of two-photon fluorescence and optical diffraction tomography, through the synchronous control of the control subsystem, and utilizes the features of the optical diffraction tomography to be label-free, non-invasive and low in phototoxicity to solve the problem of two-photon fluorescence imaging. At the same time, two-photon fluorescence is used to calibrate the results in diffraction tomography, so as to image biological samples from morphological and chemical specificity, and the two-photon fluorescence imaging device is simple and does not require complex imaging optical paths. High resolution; the fusion of two modes based on the control subsystem realizes the parallel imaging characterization of the local specificity and the global morphology of the sample; the invention can restore the three-dimensional high-resolution refractive index distribution of the test sample through optical diffraction tomography , and at the same time co-localize the two-photon fluorescence imaging of the sample to be tested to achieve dual-modal microscopic imaging.

Description

technical field [0001] The invention relates to the technical field of micro-imaging, in particular to a dual-mode micro-imaging system and an imaging method combining two-photon fluorescence and optical diffraction tomography. Background technique [0002] Optical microscopy is an important invention in human history. After four centuries of long history, the field of microscopic imaging has been continuously expanded, and different methods have emerged one after another. Microscopic imaging technology uses the interaction of light and matter to magnify tiny objects that are difficult to observe, so as to present a microscopic scene that the human eye can distinguish. Especially in the field of biomedicine, optical microscopy, as an indispensable means of observation, reveals the process of life and promotes human exploration of life sciences. In order to be able to explain life processes in the microscopic field, the imaging capabilities of microscopic imaging technology ...

Claims

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Application Information

Patent Timeline
01 Jul 2022
Publication
CN113702288B
IPC
G01N21/01; G01N21/64; G01N21/45; G01N21/84
CPC
G01N21/01; G01N21/6402; G01N21/45; G01N21/84
Inventors
施可彬; 穆书奇