A dual-modality microscope imaging system and imaging method thereof

Abstract

The invention discloses a dual-mode microscopic imaging system and an imaging method thereof. The invention combines the dual-mode microscopic imaging system of two-photon fluorescence and optical diffraction tomography, through the synchronous control of the control subsystem, and utilizes the features of the optical diffraction tomography to be label-free, non-invasive and low in phototoxicity to solve the problem of two-photon fluorescence imaging. At the same time, two-photon fluorescence is used to calibrate the results in diffraction tomography, so as to image biological samples from morphological and chemical specificity, and the two-photon fluorescence imaging device is simple and does not require complex imaging optical paths. High resolution; the fusion of two modes based on the control subsystem realizes the parallel imaging characterization of the local specificity and the global morphology of the sample; the invention can restore the three-dimensional high-resolution refractive index distribution of the test sample through optical diffraction tomography , and at the same time co-localize the two-photon fluorescence imaging of the sample to be tested to achieve dual-modal microscopic imaging.

Description

technical field [0001] The invention relates to the technical field of micro-imaging, in particular to a dual-mode micro-imaging system and an imaging method combining two-photon fluorescence and optical diffraction tomography. Background technique [0002] Optical microscopy is an important invention in human history. After four centuries of long history, the field of microscopic imaging has been continuously expanded, and different methods have emerged one after another. Microscopic imaging technology uses the interaction of light and matter to magnify tiny objects that are difficult to observe, so as to present a microscopic scene that the human eye can distinguish. Especially in the field of biomedicine, optical microscopy, as an indispensable means of observation, reveals the process of life and promotes human exploration of life sciences. In order to be able to explain life processes in the microscopic field, the imaging capabilities of microscopic imaging technology ...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above existing problems in the prior art, the present invention proposes a dual-mode microscopic imaging system and its imaging method combining two-photon fluorescence and optical diffraction tomography. Through the synchronous control of the control subsystem, the optical diffraction layer is used to The characteristics of label-free, non-invasive, and low phototoxicity of analytical technology solve the problems encountered in two-photon fluorescence imaging; at the same time, two-photon fluorescence is used to calibrate the results of diffraction chromatography, so as to analyze biological samples in terms of morphology and chemical specificity. Imaging, and the two-photon fluorescence imaging device is simple and can achieve high resolution without complex imaging optical paths; based on the control subsystem, the two modes are fused to achieve parallel imaging characterization of the local specificity and global morphology of the sample

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