35 results about "Tagged particle" patented technology

A tagged object includes a main body and a plurality of coded particles. Each coded particle may have a miniature body and be configured to provide a resolvable optical emission pattern when illuminate. The plurality of coded particles may be immobilized to the main body. A method for performing oilfield monitoring may include disposing of different types of tagged objects at different locations, wherein the different types of tagged objects each comprise a plurality of coded particles. Each of the coded particles may have a miniature body containing rare earth elements configured to produce a unique optical emission pattern when illuminated. The method may include allowing an event to trigger the release of one of the different types of tagged objects from one of the different locations. In addition, the method may include identifying the released tagged objects by unique optical emission patterns, in some cases in order to determining an occurrence location of the event.

Portable systems for processing and analyzing biological or environmental samples as well as different configurations of chip-based flow cytometers are provided. The portable systems include an automated assay preparation module configured to process a sample into a fluid assay with fluorescently tagged particles and a microfluidic analysis module coupled to the fluid assay module, wherein the microfluidic analysis module includes a chip-based flow cytometer.

In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.

The present invention provides functionalized particles and methods for delivering said particles capable of crossing a physiologic barrier and exerting an effect. In one embodiment, the present invention provides a method of delivering a particle to a mammal comprising the steps of contacting a functionalized particle with a tag and introducing the functionalized and tagged particle to a mammal, wherein the functionalized portion of the particle is selected from the group consisting of acrylic acid, 2-hydroxyethyl acrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid, allylamine, carboxyl group, hydroxyl group, sulfonic group, aldehyde and amine group. The particle is a biodegradable or nodegradable polymer and less than 1.0 mm in diameter.

Arrays of spin-valve elements that can be selectively activated to trap, hold, manipulate and release magnetically tagged biological and chemical particles, including molecules and polymers. The spin-valve elements that can be selectively activated and deactivated by applying a momentary applied magnetic field thereto. The spin valve element array can be used for selectively sorting and transporting magnetic particles one particle at a time within the array. As the magnetically tagged particles are held by the spin-valve elements, application of an auxiliary magnetic field can be used to apply tension or torsion to the held particles or to move, e.g. rotate, the trapped particles. The arrays of spin-valve elements can be used in a variety of applications including drug screening, nucleic acid sequencing, structural control and analysis of RNA / DNA and protiens, medical diagnosis, and magnetic particle susceptibility and size homogenization for other medical applications.

An apparatus, and method of use of such apparatus, for detecting particles of interest that are dispersed in a fluid mix, which typically includes other particles. The apparatus includes an interrogation platform arranged to operate in harmony with an opaque member having an orifice sized to promote single-file travel of the particles there-through. A radiation source is disposed on one side of the opaque member, and a radiation detector is disposed on the other side of the opaque member. Particles of interest are tagged using antibody-binding, fluorescing molecules. Radiation from the source causes the tagged particles to fluoresce in the vicinity of, and passing through, the orifice. The resulting fluorescence is detected by the radiation detector and indicates passage of the particles of interest. One workable opaque member is advantageously included in a thin film assembly carried on a removable and disposable card that is adapted for reception in the interrogation platform.

A microfluidic flow cytometry device includes a substrate and transverse electrodes formed on the substrate. An elastomer microfluidic focusing channel system formed on the substrate focuses a sample stream onto the floor of an outlet channel that is substantially wider and taller than cells or particles of interest and that has the transverse electrodes disposed in its floor upstream of an exit site. A step in the outlet channel upstream of the transverse electrodes vertically confines sample stream flow onto the floor of the outlet channel over the transverse electrodes. Buffer inlet channels introduce a buffer stream for horizontal focusing of the sample stream into the central region of the outlet channel at the transverse electrodes. A sample inlet channel is smaller in vertical height than the buffer inlet channels for introducing a sample stream such that the buffer vertically focuses the sample stream away from the top of the outlet channel. Sensitivity of detection is good enough to conduct both qualitative and quantitative analysis. Detection and analysis circuitry can be optimized to conduct analyze real and imaginary impedance at frequencies optimized toward tagged particles and or cells, and assays are possible.

The present invention relates to the automatic analysis and identification of different species of particles in a liquid sample using both fluorescent tagging and magnification techniques. A light source at a first wavelength is used to induce the tagged particles to emit light at a second wavelength, while the image thereof is captured on a pixel array of a digital camera. A second camera can be used to capture an image of the particles illuminated at the first wavelength, which is separated from the second wavelength by an optical filter. Alternatively, a second light source emitting pulses of light at the second wavelength, which alternate with the first light source

The present invention provides a method for detecting the presence or amount of an analyte in a sample, said method comprising: a porous surface to which particles having attached thereto a binding substance, analyte and binding substance coated label particles are added. If said analyte is present in the sample an immuno- or chemical reaction occurs in the liquid phase. The separation of bound complex from unbound material is achieved by using said surface where the separation occurs mainly two dimensionally on the surface of the porous surface (e.g. grid). Interestingly, the disclosed surface enables a separation where said complexes are distributed two dimensionally on the porous surface (e.g. grid), whereas unbound materials are distributed three dimensionally. Accordingly, said surface enables both a two and a three dimensional separation.

The invention discloses a casing leakage point depth detecting method and device for an oil extraction well. According to the method, after fluid in a wellbore of the oil extraction well reaches a quiescent state, tagged particles are added into the wellbore, and the moment t when the tagged particles are added is recorded; then a decompression valve is opened to enable the annular pressure at a casing leakage point of the oil extraction well to be lower than the wellbore pressure of the oil extraction well, the purpose that the tagged particles can be sprayed out through the leakage point in a casing along with the fluid in the wellbore at a fast speed and impact the inner wall of an intermediate casing and the outer wall of an oil-string casing is ensured, and then impact signals on the casing walls are collected; the impact signals are compared and analyzed in real time through a computer, and the moment t1 in the impacting signals including the signals of impacting of the tagged particles on the casing walls is recorded and judged; and then the distance from a well mouth of the oil extraction well to the leakage point of the casing is calculated according to the formula (please see the specifications for the formula), so that quick and effective depth detecting of the casing leakage point is achieved, complexity and high cost of casing leakage point depth detecting are lowered, and assistance is provided for safe production of the oil extraction well.

In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.

An apparatus for detecting particles of interest that are dispersed in a fluid mix, which typically includes other particles. The apparatus typically is associated with an interrogation platform arranged to operate in harmony with an opaque member having an orifice sized to promote single-file travel of the particles there-through. A currently preferred embodiment includes a light pipe configured to impinge stimulation-radiation substantially transverse to a direction of fluid flow through the opaque member. Particles of interest may be tagged using antibody-binding, fluorescing molecules. Stimulation radiation from the source causes the tagged particles to undergo a Stokes-shift emission of fluorescence. The resulting fluorescence is detected by the radiation detector and indicates passage of the particles of interest. One workable opaque member is advantageously included in a thin film assembly carried on a removable and disposable card that is adapted for reception in the interrogation platform.

The present invention relates to tagged particles and the identification and characterization of particles based on their tag. In particular, the present invention relates to a method for the production of a multitude of uniquely tagged particles comprised of a range of components selected from the group consisting of carriers, cargo and surface molecules, and the identification of such particles causing a specific effect / change in a sample, such as certain tissues / cell types.

There is provided a method for manufacturing a surface enhanced Raman scattering nano-tagging particle, the method including the steps of: introducing silver nanoparticles on the surface of a silica core particle; immobilizing tagging materials and silica shell precursors on the silver nanoparticles; and forming a silica shell surrounding the silica core particle to which the tagging materials and the silica shell precursor are immobilized.

There is provided a method for manufacturing a surface enhanced Raman scattering nano-tagging particle, the method including the steps of: introducing silver nanoparticles on the surface of a silica core particle; immobilizing tagging materials and silica shell precursors on the silver nanoparticles; and forming a silica shell surrounding the silica core particle to which the tagging materials and the silica shell precursor are immobilized.

A fluorescence-assisted counting apparatus for quantitative and / or qualitative assessments of a population of fluorescently stained particles in a specimen device retaining fluorescently stained particles, wherein the apparatus includes a detector module and a light source. The detector module includes a lens, a first optical filter and a detector, wherein the detector detects a fluorescence response. The lens includes a field of view taking in at least a portion of the fluorescently stained particles within the specimen device. The light source, further including a second optical filter, shines light in a light path that encompasses at least a portion of the particles.

A magnetic separation filter has an unsupported magnetically soft material layer having a plurality of pores, and, optionally, a passivation layer disposed on the magnetically soft material layer. The magnetic separation filter may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.

According to one embodiment, a method for detecting a sample includes preparing a device for detecting a sample, the device including a measurement cassette, a first chamber formed by partitioning the cassette with a partition wall, a through-hole provided in the partition wall, a first electrode provided in the cassette, and a second electrode provided in the cassette, introducing a reagent and a sample containing a measuring object substance into the first chamber, introducing a conductive liquid into the second chamber, supplying current to the through-hole, allowing the measuring object substance whose surface is bound to and is covered by the tag particles via the capture substance in the first chamber to pass through the through-hole, and detecting presence of the measuring object substance.

An object of the present invention is to provide a kit and a method which are capable of measuring a measurement target substance in a biological sample with high precision in a measurement range from a low concentration range to a high concentration range. According to the present invention, there is provided a kit for measuring a measurement target substance in a biological sample, including a labeled particle having a first binding substance capable of binding to a measurement target substance in a biological sample, and a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance, in which the labeled particle is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle.Each symbol in Formula (1) has the meaning described in the present specification.

The present invention relates generally to the fields of cell biology and laboratory diagnostics, and particularly to general compositions and of uniquely tagged particles linked to moieties of known properties and methods of making tagged, functionalized particles. Additionally, the invention relates to methods of screening a collection of tagged functionalized particles.

The invention discloses a remote sensing image fuzzy multi-center supervised classification method and application, and the method comprises the steps: firstly segmenting an image into particles with similar sizes in a spectral space through employing a hierarchical clustering method based on an FCM method, and then carrying out the marking and classification of the particles through employing a marking sample, and dividing the particles into three types: pure particles, impure particles, and unmarked particles, wherein the center of each pure particle represents a spectrum center of each land coverage type, the spectrum diversity of each land coverage type is represented by the centers, and the centers of each type are stored by a coverage tree; and thirdly, determining the membership degree of each unmarked sample belonging to each land coverage type by the nearest pure particles. Experimental results show that the supervised classification method realizes multi-center expression of complex remote sensing data, describes diversity of remote sensing data spectrums, and further improves precision and reliability of remote sensing classification.

An apparatus comprising an ion selecting device; an individual ion counter device; and an interface device integral with the ion selecting device and downstream of an ion separating chamber of the ion selecting device. The interface device comprises a tagging particle generator and a tagging chamber. Sample gas containing ions of a selected mobility enters the tagging chamber from the ion selecting device and is exposed to uncharged neutral tagging particles from the tagging particle generator. The ions collide with the tagging particles to form a mixture of tagged ions and uncharged neutral tagging particles which is then separated in a tagged ions separator forming part of the individual ion counting device before the separated tagged ions are counted.

The present invention relates generally to the fields of cell biology and laboratory diagnostics, and particularly to general compositions and of uniquely tagged particles linked to moieties of known properties and methods of making tagged, functionalized particles. Additionally, the invention relates to methods of screening a collection of tagged functionalized particles.

The invention discloses a casing leakage point depth detecting method and device for an oil extraction well. According to the method, after fluid in a wellbore of the oil extraction well reaches a quiescent state, tagged particles are added into the wellbore, and the moment t when the tagged particles are added is recorded; then a decompression valve is opened to enable the annular pressure at a casing leakage point of the oil extraction well to be lower than the wellbore pressure of the oil extraction well, the purpose that the tagged particles can be sprayed out through the leakage point in a casing along with the fluid in the wellbore at a fast speed and impact the inner wall of an intermediate casing and the outer wall of an oil-string casing is ensured, and then impact signals on the casing walls are collected; the impact signals are compared and analyzed in real time through a computer, and the moment t1 in the impacting signals including the signals of impacting of the tagged particles on the casing walls is recorded and judged; and then the distance from a well mouth of the oil extraction well to the leakage point of the casing is calculated according to the formula (please see the specifications for the formula), so that quick and effective depth detecting of the casing leakage point is achieved, complexity and high cost of casing leakage point depth detecting are lowered, and assistance is provided for safe production of the oil extraction well.