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3 results about "Mass cytometry" patented technology
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Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry). In this approach, antibodies are conjugated with isotopically pure elements, and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argon plasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in flow cytometry by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra.
Inductively coupled plasma-based atomic analysis system and method
Inductively coupled plasma (ICP) analyzers use an ICP torch to generate plasma in which the sample is atomized and ionized. Analysis of the atomic ions can be performed using atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle-based ICP analysis involves analyzing particles (e.g., cells, magnetic beads, or laser ablation plumes) by atomizing and ionizing them in an ICP torch chamber followed by atomic analysis. In mass cytometry, the mass tags of the particles are analyzed using mass spectrometry, such as ICP-MS. The systems and methods of this application include one or more of the following: a removable ICP torch holder assembly, an external ignition device, an ICP induction coil including annular fins, a particle-suspended sample introduction jet, and its ICP analyzer.
Owner:FLUIDIGM CANADA INC
Mass tags for mass cytometry and methods of making and using the same
This invention provides a mass tag for mass flow cytometry, its preparation method, and its application. The mass tag provided by this invention has the chemical structure shown in Formula 1. The compound shown in Formula 1 provided by this invention offers a new channel for CyTOF single-cell analysis, suitable for the detection of various biomarkers such as live cells and proteins. It is of great significance for more in-depth and comprehensive analysis of changes in various cell subtypes and their proportions, and for predicting the changing trends of clinical disease courses, showing good clinical application prospects and significant social benefits.
Owner:PEKING UNIV
Mass cytometry reagents and methods for signal amplification
Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to the mass tags, and additional reagents to reduce background and / or preserve specific binding partner (SBP) binding of the mass label, as well as protocols for associating multiple mass tags with a single SBP. Thus, embodiments include any combination of one or more reagents and uses thereof. The reagents, kits, and methods herein are useful in mass cytometry, including imaging mass cytometry. In some aspects, the reagents, kits, or methods are useful for delivering a large number of radioisotopes to a target analyte, e.g., for therapeutic use or radioimaging. In certain aspects, only non-radioactive isotopes are used in mass cytometry.
Owner:STANDARD BIOTOOLS CANADA INC