A Method for Removing Gadolinium Isotope Channel Contamination in Mass Cytometry Data

A flow data and isotope technology, applied in the field of mass spectrometry and flow cytometry, can solve problems such as biased analysis conclusions, and achieve the effect of improving quality

Active Publication Date: 2022-06-07
ZHEJIANG UNIV
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Problems solved by technology

This approach will directly change the composition of the cells in the tested sample, which is prone to biased analysis conclusions

Method used

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  • A Method for Removing Gadolinium Isotope Channel Contamination in Mass Cytometry Data
  • A Method for Removing Gadolinium Isotope Channel Contamination in Mass Cytometry Data
  • A Method for Removing Gadolinium Isotope Channel Contamination in Mass Cytometry Data

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Embodiment 1

[0085] First, a gadolinium isotope-contaminated sample was stained with gadolinium isotope channel uncoupled antibody and detected by CyTOF on the computer. The obtained 155Gd~158Gd, 160Gd channel detection data were used as gadolinium contamination signals and superimposed on a sample data without gadolinium isotope channel contamination. (raw, without gadolinium isotope contamination), generate a synthetic simulation data (simulate, with gadolinium isotope contamination) that has the characteristics of gadolinium isotope collinearity and pairwise strong correlation between channels ( image 3 A and B).

[0086] Using R provided in the present invention Gd Estimation method (Equation (4)), obtaining a pair of R Gd The estimates are as follows:

[0087] R Gd = [1.000000, 1.308478, 1.004611, 1.764121, 1.714435].

[0088] Then use formula (5)-formula (9) to estimate the k value, and then use formula (3) to correct the pollution data, and obtain the correction data correspond...

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Abstract

The invention discloses a method for removing gadolinium isotope channel pollution in mass spectrometry data. By using the gadolinium isotope channel ( 155 Gd~ 158 Gd, 160 Gd) Contamination signals have collinear characteristics, and under the condition that pre-designed gadolinium isotope-coupled antibody-specific labeled biomarkers cannot be co-expressed on a single cell at the same time, calculate the estimation of contamination signal of a single cell in the gadolinium isotope channel value, and obtain data corrected to remove gadolinium isotope channel contamination. The present invention provides a method for estimating and removing the pollution signal of the gadolinium isotope channel in the mass spectrometry flow data, which avoids the deviation caused by the artificial change of the cell components of the tested sample brought about by directly removing the polluted cells, It has important application value for improving the quality of mass spectrometry flow detection data and subsequent data analysis.

Description

technical field [0001] The invention relates to the technical field of mass flow cytometry, in particular to a method for removing contamination of gadolinium isotope channels in mass flow cytometry data. Background technique [0002] Gadolinium (Gd) isotope channel contamination is commonly seen in mass cytometry data obtained by using mass cytometry to detect local tissue samples from patients. [0003] Mass Cytometry (CyTOF for short) is a single-cell, high-throughput, high-dimensional detection technology. Accurate quantitative detection of the calibrated rare metal content is carried out by using the principle of mass spectrometry. Compared with traditional flow cytometry, it has the characteristics of higher throughput and higher detection signal precision. Currently, there are more than 40 channels of rare metals used to couple antibodies, among which the gadolinium isotope channel ( 155 Gd~ 158 Gd, 160 Gd) is a common channel for CyTOF detection. [0004] Gadol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N27/62G01N1/34
CPCG01N15/14G01N27/62G01N1/34
Inventor 尹巍巍刘俊伟陈伟
Owner ZHEJIANG UNIV
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