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Method for screening biomarker related to degree of fatigue in human body fluid by liquid chromatography-mass spectrometry

A biomarker, fatigue level technology, used in instruments, measuring devices, scientific instruments, etc.

Active Publication Date: 2019-03-05
中国民用航空局民用航空医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is still a lack of methods that can be used to screen biomarkers related to human fatigue

Method used

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  • Method for screening biomarker related to degree of fatigue in human body fluid by liquid chromatography-mass spectrometry
  • Method for screening biomarker related to degree of fatigue in human body fluid by liquid chromatography-mass spectrometry
  • Method for screening biomarker related to degree of fatigue in human body fluid by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] Example 1 Screening method for biomarkers of fatigue degree in body fluids of simulated civil aviation aircraft pilots operating for 3.5 hours

[0124] 1.1 Volunteer recruitment and sample collection:

[0125] Recruit 99 volunteers. Enrollment conditions: healthy, no medication, aged 20-55, including 55 males and 44 females. Female volunteers need not be in menstruation.

[0126] Arrange volunteers to simulate aircraft driving operation for 3.5 hours continuously to induce mild fatigue.

[0127] Collection of blood and urine samples: Blood and urine samples were collected before and after the simulated driving operation of the volunteers. Blood samples were collected with anticoagulant blood collection tubes to collect no less than 2.0 mL of venous blood, and urine samples were collected with sterile urine cups. solution and stored in sterile tubes.

[0128] Human blood and urine samples are preserved, the blood is centrifuged at 4000-5000rpm for 3-5min, the upper pla...

Embodiment 2

[0150] Example 2 Screening method for fatigue biomarkers in body fluids of traffic controllers in civil aviation

[0151] 2.1 Volunteer recruitment and sample collection:

[0152] Recruitment of volunteers: Recruit 45 civil aviation traffic controller volunteers at an international airport in China. Enrollment conditions: healthy, male, no medication, age 20-35, divided into 2 groups, the first group (ATC1) 20 people, the second group (ATC2) 25 people. At the same time, 23 volunteers of the airport's administrative and logistics personnel were recruited as the control group (CON). The entry conditions were: healthy, male, no medication, aged 20-35 years old.

[0153] Traffic controllers in civil aviation are responsible for commanding the safe operation of aircraft on air traffic routes. In layman's terms, they are commanding aircraft and coordinating and managing all aircraft in the entire airspace with reference to radar and other information. At work, controllers often co...

Embodiment 3

[0185] Example 3 Screening method for biomarkers of fatigue degree in body fluids of simulated civil aviation aircraft pilots operating for 1 hour

[0186] 3.1 Volunteer recruitment and sample collection:

[0187] Recruit 20 volunteers, enrollment conditions: healthy, no medication, aged 20-40, including 10 males and 10 females, female volunteers need not be in menstruation.

[0188] Volunteers were arranged to perform simulated aircraft driving operations for 1 hour without fatigue.

[0189] Urine sample collection: Urine samples were collected before and after the volunteers performed the simulated driving operation. Urine samples were collected using sterile urine cups and stored in sterile tubes.

[0190] Human urine samples are preserved, and the urine samples are divided into sterile centrifuge tubes and stored at -80°C.

[0191] 2.2 Pretreatment of human urine samples:

[0192] The urine sample pretreatment steps are as follows: take out the urine sample and place it...

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Abstract

The invention relates to a method for screening a biomarker related to the degree of fatigue in human body fluid by liquid chromatography-mass spectrometry. The method comprises the following steps: step 1, collecting human body fluid samples in different groups; step 2, pretreating the samples collected in step 1; step 3, detecting the samples pretreated in step 2 by the liquid chromatography-mass spectrometry; step 4, processing data obtained by detection in step 3 and setting threshold values, and screening out potential biomarkers related to the degree of fatigue; step 5, carrying out metabolic pathway retrieval on the potential biomarkers related to the degree of fatigue screened in step 4 and confirming the structure of the potential biomarker; and step 6, comparing structure-confirmed compounds in different groups in step 5, and confirming the biomarkers related to the degree of fatigue. The screening method is good in universality and high in accuracy and confidence level.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for screening biomarkers related to fatigue degree in human body fluids by liquid chromatography-mass spectrometry. Background technique [0002] The history of human research on fatigue can be traced back to the First World War a hundred years ago. During the war and after the war, the British Industrial Fatigue Research Board (The Industrial Fatigue Research Board, later renamed The Industrial Health Research Board) carried out a study on industrial production, especially military supplies. A large-scale ergonomic study of fatigue problems in material production. The research report has a far-reaching impact, and even many results have become the consensus of the industry. For example, the report recommends reducing excessive working hours, improving the working environment, and adjusting the shift system to alleviate employee fatigue and incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈振玲刘永锁李丽丽姜薇徐先发许海山王伟王妍刘铁兵王雷朱安林杨剑
Owner 中国民用航空局民用航空医学中心
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