The invention relates to the technical field of biotechnology, in particular to a multi-RT-PCR detection primer set for TGEV, PEDV, SADS-CoV and PDCoV. The nucleotide sequence of the multi-RT-PCR detection primer set for TGEV, PEDV, SADS-CoV and PDCoV is shown as SEQ ID NO. 1-8. The invention also provides a kit comprising the primer set. The primer set and the kit adopt one-time PCR reaction. TGEV, PEDV, SADS-CoV and PDCoV can be identified rapidly. The specificity is good, the sensitivity is high, the repeatability and stability are good, and the primer set is suitable for differential diagnosis of TGEV, PEDV, SADS-CoV and PDCoV. The multi-PCR kit is quite suitable for pathogen diagnosis, pathogen purification detection and epidemiological investigation of epidemic diseases such as a laboratory, an entry and exit site and a farm.