A method for screening traditional Chinese medicine monomers

A traditional Chinese medicine, monomer technology, applied in the field of molecular biology, can solve problems such as liver cell damage

Inactive Publication Date: 2012-10-17
FUDAN UNIV +1
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AI Technical Summary

Problems solved by technology

Studies have shown that hepatic cell apoptosis in the early stage of acute liver injury or liver fibrosis can inhibit the occurrence of hepatic fibrosis, but in the advanced stage of fibrosis after HSC activation, the apoptosis of hepatocytes will cause continuous damage to hepatocytes , a large number of activation and proliferation of HSC, and then promote the further development of liver fibrosis

Method used

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  • A method for screening traditional Chinese medicine monomers
  • A method for screening traditional Chinese medicine monomers
  • A method for screening traditional Chinese medicine monomers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] (1) Establishment of cell oxidative damage model and grouping of traditional Chinese medicine monomer effects

[0077] 1. Main reagents:

[0078] High glucose medium (DMEM) was purchased from Invitrogen Company, and calf serum was purchased from Shanghai Shisheng Company. Salvianolic acid B, danshensu sodium, protocatechualdehyde, cordyceps polysaccharide, cordycepin, cordycepic acid and other traditional Chinese medicine monomers were provided by Shanghai University of Traditional Chinese Medicine.

[0079] 2. Experimental steps:

[0080] Take the H7702 cells in the logarithmic growth phase, make a single cell suspension, and use 5×10 4 Cells / ml were inoculated into 96-well culture plates, and cultured in 200 μl of culture medium at 37° C. and 5% CO2 incubator for 12 hours. Join H 2 o 2 Make the final concentration 400μM, continue to culture for 2h, and establish the oxidative damage model.

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Abstract

The invention belongs to the technical field of molecular biology, and relates to a method for screening traditional Chinese medicine monomers, specifically relating to a method for screening monomer ingredients which have protective effect for oxidative damages to liver cells from Chinese traditional medicines by using mitochondrion index technique. In the invention, mitochondrial function detection is used as the core technology and methods of cell proliferation detection, mitochondrial reactive oxygen species generation detection, mitochondrial membrane potential detection, energy substance ATP in mitochondria detection and mitochondrial cytochrome C detection are adopted. Mitochondrial function changes are detected after H7702 cells, which are subject to oxidative damages induced by H2O2, are treated by the traditional Chinese medicine monomers of SalB, protocatechualdehyde, sodium danshensu, cordyceps polysaccharose, cordycepin and cordycepic acid, and the traditional Chinese medicine monomers, which have protective effect for oxidative damages to liver cells, are screened. The invention further provides applications of the traditional Chinese medicine monomers screened in preparing drugs for preventing and treating liver diseases such as fatty liver, viral hepatic diseases and liver fibrosis.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a method for screening traditional Chinese medicine monomers, in particular to a method for screening traditional Chinese medicine monomers using mitochondrial index technology, especially a method for screening traditional Chinese medicine monomers that resist oxidative damage to liver cells Background technique [0002] Studies have revealed that oxidative damage to liver cells is the pathological basis of diseases such as liver fibrosis. In this process, the energy center in cells, mitochondrial function changes, and thus mediate the process of apoptosis. Mitochondria are known to be important organelles of eukaryotic cells, not only the energy center of animal cells, the main site for generating ATP, but also the main switch in apoptosis. The tricarboxylic acid cycle enzyme system of the mitochondrial matrix generates NADH through the dehydrogenation and oxidation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15A61K31/343A61K31/11A61K31/192A61K31/736A61K31/7076A61K31/047A61P1/16
Inventor 刘雯刘平左伋刘云霞刘成海
Owner FUDAN UNIV
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