Primer and probe for real-time fluorescence PCR (polymerase chain reaction) detection of goose down component

A real-time fluorescence and probe technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high subjectivity, high requirements for inspectors, and easy misjudgment, and achieve Easy operation, high sensitivity and strong specificity

Inactive Publication Date: 2015-05-20
中华人民共和国辽宁出入境检验检疫局 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These standards only qualitatively explain the structural characteristics of down, and it is difficult to effectively guide the identification of duck down and goose down.
The method has high requirements for inspectors, is highly subjective, and is prone to misjudgment

Method used

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  • Primer and probe for real-time fluorescence PCR (polymerase chain reaction) detection of goose down component
  • Primer and probe for real-time fluorescence PCR (polymerase chain reaction) detection of goose down component
  • Primer and probe for real-time fluorescence PCR (polymerase chain reaction) detection of goose down component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Take samples from unused parts of down jackets or other down products, and take a total of about 1g of samples. Cut the sample into pieces with scissors, disperse and mix them with an analytical grinder, then cut them into pieces with scissors as much as possible to less than 2mm, and mix them with an analytical grinder again.

[0037] 2. Weigh about 5 mg of sample from the mixed sample, put it into a 2mL centrifuge tube, extract 2 tubes in parallel for each sample, add 180 μL of buffer GL (Buffer GL), 20 μL of proteinase K (Proteinase K) and 10 μL of ribonuclease A (RNase A, 10 mg / mL), warmed in a 56°C water bath until the tissue was completely lysed for 3 hours. If the remaining fibrous tissue cannot be completely lysed, centrifuge at 12,000 rpm for 2 minutes after lysing to remove impurities before performing subsequent operations. Add 200 μL of Buffer GB (Buffer GB) and 200 μL of 100% ethanol to the lysate, and mix well by pipetting. During the warm bath, the s...

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PUM

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Abstract

The invention belongs to a qualitative detection technology of animal-derived ingredients in feather down products and specifically relates to a primer / probe group for detecting a goose down component in the feather down product. A group of specific primer and probe is designed by selecting a mitochondrial cytochrome B (CYTB) gene sequence of goose down. By using the primer and the probe and adopting a real-time fluorescence PCR (polymerase chain reaction) technology, the goose down component in the feather down product can be fast, sensitively and specifically detected. The primer and the probe can be provided together with other reagents in the form of a kit and be used for nucleic acid amplification reaction. The method is simple and convenient to operate and good in repeatability.

Description

technical field [0001] The invention relates to a method for rapid detection of animal-derived components using nucleic acid amplification technology, in particular to a primer and probe sequence for real-time fluorescent PCR detection of goose down components. Background technique [0002] Down is the fluff that grows on the abdomen of geese and ducks, and is called feathers in flakes. Because down is a kind of animal protein fiber, it has higher thermal insulation than cotton, wool, and silk, and the down ball-shaped fiber is densely covered with tens of thousands of small triangular pores, which can shrink and expand with temperature changes, and have the function of temperature regulation. Absorb the flowing hot air from the human body and isolate the invasion of cold air from the outside. Therefore, down is an excellent filler and has always been welcomed by people at home and abroad. As the world's largest producer, exporter and consumer of down and feather products,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2561/113C12Q2561/101C12Q2563/107
Inventor 王玫任亮孔平杨春光颜怀玉肇慧君李晶泉张浩
Owner 中华人民共和国辽宁出入境检验检疫局
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