Primers and probes used for detecting ingredients of racoon dog origin, and detection method of ingredients of racoon dog origin

A technology for component detection and raccoon dog origin, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The identification and identification of raccoon-derived components have achieved the effect of low test sample requirements, reduced impurity interference, and rapid identification

A technology for component detection and raccoon dog origin, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The identification and identification of raccoon-derived components have achieved the effect of low test sample requirements, reduced impurity interference, and rapid identification

CN103602732AActive Publication Date: 2014-02-26INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Primers and probes used for detecting ingredients of racoon dog origin, and detection method of ingredients of racoon dog origin
  • Primers and probes used for detecting ingredients of racoon dog origin, and detection method of ingredients of racoon dog origin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] (1) Design of specific primers for real-time fluorescent PCR detection method

[0020] Download the mitochondrial gene sequences of various animals published in GenBank, and use DNAMAN software to compare and analyze, select the mitochondrial cytochrome oxidase b gene sequence of raccoon dogs to design specific fluorescent PCR primers and probes for identifying and detecting raccoon-derived components (only The restriction fragments of raccoon DNA can be amplified, but the restriction fragments of other animal DNA cannot be amplified), the primers and probes are as follows:

[0021] Upstream primer sequence: 5'-TACAGCTGATCTCCTCACGTTAACA-3';

[0022] Downstream primer sequence: 5'-TTGGCCGATGATGATGAAAG-3';

[0023] Probe sequence: 5'(FAM)-AATTGCAGGCCAACCGGTCGAAC-3'(TAMRA).

[0024] (2), DNA extraction

[0025] (2.1) Take the sample and grind it into minced or finely ground by a grinder, and dry it in a constant temperature drying oven. Samples with high fat content suc...

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Abstract

The invention relates to qualitative detection methods of ingredients of animal origin in food, forage, fur, and products of food, forage and fur, and more specifically relates to primers and probes used for detecting ingredients of racoon dog origin, and a detection method of the ingredients of racoon dog origin. According to the detection method, gene sequences of mitochondrion cytochrome oxidase b are selected, a set of specific primers and probes is designed, and the specific primers and probes are used for real-time fluorescent PCR, so that simple, convenient and fast detection of the ingredients of racoon dog origin in food, forage, fur, and the products of food, forage and fur is realized.

Description

technical field [0001] The invention relates to the detection of animal-derived components, in particular to a primer and probe for detecting raccoon-raccoon-derived components and a detection method thereof. Background technique [0002] The breeding scale of raccoon dogs continues to expand, and the fur products of raccoon dogs are very marketable. At the same time, the breeding volume of raccoon dogs is large, and the individual raccoon dogs are large. As a by-product of the raccoon fur industry, raccoon dog meat is often added to food or feed. Therefore, it is necessary to provide a method that can quickly and accurately detect raccoon-derived components. Traditional sensory methods to identify raccoon-derived components are judged based on color, smell, and tissue properties, which are easily affected by subjective factors and require the accumulation of experience. The difficulty of identification and the risk of monitoring supervision. At present, there is still no...

Claims

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Application Information

Patent Timeline
26 Feb 2014
Publication
CN103602732A
IPC
C12Q1/68; C12N15/11
CPC
C12Q1/686; C12Q2531/113; C12Q2561/113; C12Q2563/107
Inventors
钱云开; 王海洋