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Method for identifying Gaoyou duck varieties by utilizing molecular bioinformatics

A technology of molecular biology and Gaoyou duck, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of high cost, long identification time, and low accuracy

Inactive Publication Date: 2010-08-25
JIANGSU INST OF POULTRY SCI
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Problems solved by technology

[0004] The purpose of the present invention is to check the breeding files based on the morphological markers, measure the production performance and use biochemical markers such as isozymes and proteins to identify varieties, such as low accuracy, long identification time, and high costs. , to provide a method for identifying Gaoyou duck breeds using molecular bioinformatics to achieve accurate and rapid identification of Gaoyou duck breeds, which is conducive to safeguarding the interests of relevant personnel

Method used

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Embodiment Construction

[0007] Further illustrate the present invention in conjunction with embodiment, its method is:

[0008] (1) 30 female ducks were randomly selected from the tested laying duck species and Gaoyou duck, and blood was collected from wing veins, and the total DNA including mitochondrial DNA was extracted from the blood samples by the phenol-chloroform method.

[0009] (2) Design a pair of primers according to the mallard duck mtDNA cytochrome b gene sequence (NC009684) published on GeneBank, the primer sequence is:

[0010] Upstream primer 5'-GTAGCCGCAAGCAAAG-3';

[0011] Downstream primer 5'-GGGAGCTGGAGATAAAGG-3'.

[0012] (3) The designed primers were used to amplify the mtDNA cytochrome b gene of Gaoyou duck and tested duck species by PCR. The PCR products were cloned and sequenced on ABI3730 sequencing instrument.

[0013] (4) The sequencing results were read by Chromas1.49 software, and the sequencing results were manually read base by base and checked repeatedly to ensure a...

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Abstract

The invention relates to a method for identifying Gaoyou duck varieties by utilizing molecular bioinformatics, belonging to the technical filed of biological variety identification. In the invention, the sequences of gene b of cytochrome of mitochondrial DNA of a Gaoyou duck and the duck variety to be tested are accurately obtained on the basis of gene cloning and DNA sequencing technologies, a plurality of bioinformatics software is utilized to accurately figure out the haplotype, variation locus, haplotype diversity, average nucleotide difference, nucleotide ramification degree, pure genetic distance, Kimura dual-parameter genetic distance among varieties, variety colony cluster result images and haplotype phylogenetic trees of each variety, and the genetic information difference among the varieties is utilized to identify the true and false of the Gaoyou duck varieties through software precise calculation and analysis results.

Description

technical field [0001] The invention relates to a method for identifying Gaoyou duck varieties by using molecular bioinformatics, and belongs to the technical field of biological variety identification. Background technique [0002] The traditional Gaoyou duck and Gaoyou double-yellow duck eggs are well-known at home and abroad, and they are the unique resources of Gaoyou. However, due to the lack of attention to the establishment of the original breed base, the formulation of brand standards, and the protection of patents, some counterfeit products have occupied the market and reduced the reputation of traditional products. . It is not uncommon for some unscrupulous companies and farmers to illegally sell counterfeit and counterfeit Gaoyou ducks in the market, which seriously damages the reputation of Gaoyou ducks and affects the development of leading enterprises of Gaoyou duck group. [0003] At present, the identification of poultry breeds is mainly based on morphologic...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李慧芳朱文奇陈宽维束婧婷宋卫涛韩威徐文娟汤青萍章明
Owner JIANGSU INST OF POULTRY SCI
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