Difunctional nanoprobe for detecting mitochondrial cytochrome C as well as preparation method thereof
A technology of cytochrome and nanoprobe, applied in the field of magnetic resonance imaging, can solve the problems of large rejection of organisms and poor biocompatibility of nanoparticles
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Embodiment 1
[0029] This embodiment provides a method for preparing a bifunctional nanoprobe for detecting mitochondrial cytochrome C, comprising the following steps:
[0030] Step 101: Take the ligand of mitochondrial cytochrome C, and label the two ends with fluorescein and aldehyde respectively to obtain the first product;
[0031] Step 102: mixing the first product with magnetosomes and incubating overnight, then removing unreacted substances to obtain a second product;
[0032] Step 103: Add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide and methoxy-carboxylated-polyethylene diimide to the second product Alcohol, and incubated overnight, after removing unreacted substances, a dual-functional nanoprobe for detecting mitochondrial cytochrome c was obtained.
[0033] The reaction principle of the above method is:
[0034] Before the preparation of the probe, it has been known through characterization that the magnetosome is a ferromagnetic chain-like na...
Embodiment 2
[0044] This embodiment provides a method for preparing a bifunctional nanoprobe for detecting mitochondrial cytochrome C, comprising the following steps:
[0045] Step 201: Take the ligand of mitochondrial cytochrome C, and label the two ends with fluorescein and aldehyde respectively to obtain the first product;
[0046] Step 202: Mix the first product with magnetosomes at a ratio of 3:7 (mass ratio), incubate overnight, and then use a magnet to absorb the mixture to obtain an adsorbate, and then rinse the adsorbate to obtain a second product; wherein, the incubated The conditions are: under the condition of avoiding light, sonicate for 20s every 50-200s, and the power of the sonication is 20-80w.
[0047] Step 203: Add EDC, NHS and H to the second product 3 C-O-PEG-COOH, incubate overnight, and then use a magnet to absorb the mixture to obtain the adsorbate, and then rinse the adsorbate to obtain a dual-functional nanoprobe for detecting mitochondrial cytochrome C; the incu...
Embodiment 3
[0053] This embodiment provides a bifunctional nanoprobe for detecting mitochondrial cytochrome C, which can be prepared by any of the above methods.
[0054] In addition, in order to further illustrate the technical effects of the present invention, specific test examples are also provided below.
[0055] In this test example, for convenience of description, the bifunctional nanoprobe for detecting mitochondrial cytochrome C of the present invention is referred to as "PEG-cBMP" for short.
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