Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA molecule identification method of scorpion medicinal materials in Naoxintong preparation

A technology of DNA molecules and brain-to-heart communication, which is applied in the field of DNA molecular identification to achieve the effect of improving quality control and perfecting quality standards

Pending Publication Date: 2019-07-26
SUN YAT SEN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, most of the above studies use the general primers of COI, 12S rRNA and 18S rRNA genes to perform PCR on the DNA of medicinal materials, and the size of the amplified target product fragment is about 350-700bp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA molecule identification method of scorpion medicinal materials in Naoxintong preparation
  • DNA molecule identification method of scorpion medicinal materials in Naoxintong preparation
  • DNA molecule identification method of scorpion medicinal materials in Naoxintong preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of DNA molecular identification method of the scorpion medicinal material of embodiment 1

[0036] 1. Experimental samples

[0037] The scorpion experimental samples used in the examples are shown in Table 1. The identification of each sample in the table is by using the COI universal primer HCO2198 / LCO1490 (HCO2198: 5'-TAAACTTCAGGGTGACCAA AAAATCA-3', LCO1490: 5'-GGTCAACAAATCATAAAGATATTGG-3' ) After performing PCR amplification and sequencing, BLAST is performed, and the species corresponding to the sequence with the highest similarity to the sequence to be tested is the species closest to the sample to be tested.

[0038] Table 1 Sources of commercially available Scorpio products

[0039]

[0040] 2. Extraction of total DNA from scorpions to be identified

[0041] Take 20-50 mg of muscle tissue from scorpion medicinal materials in a sterilized 1.5mL centrifuge tube, cut up the scorpion tissue with sterilized small scissors, and use the tissue / cell genomic D...

Embodiment 2

[0056] Embodiment 2 A kind of DNA molecular identification method of the scorpion medicinal material in Naoxintong capsule

[0057] 1. Experimental samples

[0058] 9 batches of Naoxintong Capsules (batch numbers: 171275, 171278, 1712103, 1712104, 1712105, 180478, 180585, 180655, 180721), all from Shaanxi Buchang Pharmaceutical Co., Ltd., respectively numbered NXT-1~9.

[0059] 2. Total DNA extraction method of Naoxintong capsules to be identified

[0060] The broad-spectrum plant genomic DNA rapid extraction kit (Biomed, Beijing, China) was used to extract the herbal drug components in the capsules, and proteinase K was added during the process to digest the animal drug components in the capsules. The specific steps are: take about 50mg of Naoxintong capsule fine powder into a 1.5mL sterilized centrifuge tube, add 400μL buffer AP1 and 20μL proteinase K (20mg / mL, Biomed, China), vortex, mix well and place Water bath at 55°C overnight; the next steps are the same as those in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA molecule identification method of scorpion medicinal materials in a Naoxintong preparation. According to the method, animal medicine components are digested by proteinaseK, and the plant components are extracted by a broad-spectrum plant genome DNA rapid extraction kit. Scorpion specific primers are designed based on mitochondrial cytochrome c oxidase subunit I (COI)for PCR amplification; and electrophoresis sequencing is conducted on the product, and result judgment is conducted on the obtained sequence in a GenBank database by using BLAST, wherein the speciescorresponding to the sequence with the highest similarity is the species closest to the sample to be detected. The specific primers used in the method can amplify a small fragment gene of about 250 bp, is not limited by the external form of scorpion, can identify a traditional Chinese medicine preparation containing scorpion powder, especially Naoxintong capsules, avoids the influence caused by DNA degradation, and has the characteristics of accuracy and universality. The quality control level of animal scorpion medicinal materials is improved, and the quality standard of Naoxintong capsules is further improved.

Description

technical field [0001] The invention relates to a method for DNA molecular identification of scorpion medicinal materials in Naoxintong preparation. Background technique [0002] Naoxintong Capsule is an exclusive single-product regulated Chinese medicine variety of Shaanxi Buchang Pharmaceutical Co., Ltd. (Z20025001, specification: 0.4g per capsule), which is composed of Astragalus, Salvia, Angelica, Radix Paeoniae Rubra, Chuanxiong, peach kernel, safflower , Vinegar Frankincense, Vinegar Myrrh, Caulis Spatholobus, Achyranthes bidentata, Guizhi, Morangzhi, Dilong, Leech, Scorpion and other 16 traditional Chinese medicines are directly powdered and pharmaceutically prepared. It is used for the treatment of stroke, hemiplegia, numbness of limbs, crooked mouth and eyes, strong tongue, lingering speech, chest pain, chest tightness, palpitation, shortness of breath, cerebral infarction, coronary heart disease and angina pectoris caused by qi deficiency and blood stagnation, vena...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2535/122C12Q2565/125C12Q2537/165
Inventor 苏薇薇朱晓枭吴灏
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com