DNA bar code, primer pair, kit and method for identifying aquatic coleoptera Mycoiliaceae Mycoiliaceae Mycoiliaceae Mycoiliaceae insects

A Coleoptera, primer pair technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA/RNA fragments, etc., can solve problems such as difficulty in morphological identification, shorten the identification cycle, save time, and do not require professional knowledge. high effect
CN112795664APending Publication Date: 2021-05-14LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
Publication Date
2021-05-14

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Abstract

The invention belongs to the field of species identification, and particularly relates to a DNA bar code, a primer pair, a kit and a method for identifying aquatic coleoptera Mycoiliaceae Mycoiliaceae Oriental Mycoiliaceae insects, and the DNA bar code is a mitochondrial cytochrome c oxidase I gene (CO I); the universal primer sequence of the gene is SEQ ID NO. 1. Through polymerase chain reaction (PCR) specific amplification, agarose electrophoretic separation, gene fragment sequencing and sequence para-position arrangement analysis, if the comparison homology of the gene sequence is 98% or above, it can be judged that the to-be-detected insects are of the same type, and otherwise, it can be judged that the to-be-detected insects are of different types. According to the specific DNA sequence of the Mycoiliaceae Oriental Mycoiliaceae and the identification method of the Mycoiliaceae Oriental Mycoiliaceae, the defects of traditional morphological identification are overcome, and rapid and accurate identification of different insect states and incomplete specimens of Mycoiliaceae insects is facilitated.
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Claims

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