DNA bar code, primer pair, kit and method for identifying aquatic coleoptera Mycoiliaceae Mycoiliaceae Mycoiliaceae Mycoiliaceae insects

Abstract

The invention belongs to the field of species identification, and particularly relates to a DNA bar code, a primer pair, a kit and a method for identifying aquatic coleoptera Mycoiliaceae Mycoiliaceae Oriental Mycoiliaceae insects, and the DNA bar code is a mitochondrial cytochrome c oxidase I gene (CO I); the universal primer sequence of the gene is SEQ ID NO. 1. Through polymerase chain reaction (PCR) specific amplification, agarose electrophoretic separation, gene fragment sequencing and sequence para-position arrangement analysis, if the comparison homology of the gene sequence is 98% or above, it can be judged that the to-be-detected insects are of the same type, and otherwise, it can be judged that the to-be-detected insects are of different types. According to the specific DNA sequence of the Mycoiliaceae Oriental Mycoiliaceae and the identification method of the Mycoiliaceae Oriental Mycoiliaceae, the defects of traditional morphological identification are overcome, and rapid and accurate identification of different insect states and incomplete specimens of Mycoiliaceae insects is facilitated.

Description

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AI Technical Summary

Benefits of technology

This technology helps detect differences between individuals' unique characteristics such as their body shape or features like eyesight or skin coloration by comparing them with existing data from previous generations that have been collected for each person. It also saves on labor costs compared to current methods requiring advanced technologies involving complicated procedures.

Problems solved by technology

The technical problem addressed in this patents relates to finding ways to quickly and accurately detect small amounts of slender rodys from various habitats around Japan's mainland where these pest populations live throughout their entire life cycle. Current methods require extensive effort due to variations between individuals and environmental factors like temperature fluctuation.

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