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Specific primer for amplifying aleyrodidae insect mitochondrial COI gene

A technology of whiteflies and mitochondria, applied in the field of applied biology, can solve the problems of incomplete preservation, difficulty in morphological identification, loss, etc., and achieve the effects of shortening the experimental cycle, good sequencing peaks, and reducing experimental costs

Inactive Publication Date: 2015-10-21
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Indirect damage: Adults and nymphs secrete honeydew and waxy substances to pollute plant organs and fruits, induce the occurrence of soot disease, hinder plant photosynthesis, cause leaves to shrink, wither and fall leaves early, and at the same time reduce the quality and quality of crops. decline
(3) Some whiteflies are important vectors of many viral diseases, and the plant viruses transmitted can cause plant deformities and fruit abortion, causing serious losses
Therefore, for whitefly pests stored in alcohol collected in different places, the collected whiteflies are adults, or incompletely preserved specimens, it is very difficult to identify them only through morphological identification.

Method used

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  • Specific primer for amplifying aleyrodidae insect mitochondrial COI gene
  • Specific primer for amplifying aleyrodidae insect mitochondrial COI gene
  • Specific primer for amplifying aleyrodidae insect mitochondrial COI gene

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Embodiment Construction

[0015] The method for screening the amplification primers of the COI gene of the whitefly family of the present invention is divided into two steps: 1. Based on the sequence of the COI gene in the mitochondrial genome sequence of the whitefly family insects that has been measured, the COI gene is found by comparison Relatively conservative sequence fragments, design primers; 2. Amplify the synthesized primers to the COI gene of the whitefly family, and test its versatility and amplification efficiency in the family whiteflies.

[0016] The invention will be further explained below with examples:

[0017] 1. Sample collection: Specimens collected in Jiangsu, Zhejiang, Fujian, Jiangxi, Shaanxi, Hubei, Guangxi, Guangdong, Hainan and other provinces of my country from 2010 to 2014.

[0018] 2. DNA extraction: use a dissecting needle to pick up adult Bemisia tabaci, wash thoroughly with ultrapure water (ddH2O) and place in a 1.5mL centrifuge tube, add 5μL of extraction lysate (1% SDS, 500...

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Abstract

The invention relates to a specific primer for amplifying the gene sequence of mitochondrial cytochrome c oxidase I (COI) of aleyrodidae insects. The sequence is represented by the SEQ ID No.1 and SEQ ID No.2. The provided amplification primer can rapidly and effectively amplify COI genes of multiple species of aleyrodidae insects, and thus can provide reliable references for the researches on growth relationship of aleyrodidae system, and population genetic differentials and genetic structure of specific insects.

Description

Technical field [0001] The invention belongs to the field of applied biotechnology, and specifically relates to a pair of specific primers for amplifying the mitochondrial cytochrome c oxidase I (COI) gene of the whitefly family. Background technique [0002] Aleyrodidae (Aleyrodidae) belongs to Hemiptera and Sternorrhyncha. Whitefly insects are distributed in major animal areas in the world, host plants are extensive, and some species are important agricultural pests. For example, Bemisia tabaci (Gennadius), a major agricultural pest, is widely distributed throughout the world, causing great economic losses to agricultural production. [0003] In my country, the whiteflies that cause harm to agriculture include whitefly Bemisia tabaci, Trialeurodes vaporariorum, Trialeurodes vaporariorum, Aleurocanthus spiniferus, Dialeurodes citri, Pealius mori, and Vasdavidius indicus. In recent years, Aleurodicus dispersus has invaded Hainan. Whitefly pests mainly harm host plants in the foll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10
Inventor 杜予州汤小天王吉锐陆明星
Owner YANGZHOU UNIV
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