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Specific primers for amplifying the mitochondrial coi gene of lepidopteran insects

A lepidopteran insect and mitochondrial technology, applied in the direction of DNA preparation, DNA / RNA fragments, recombinant DNA technology, etc., to achieve the effect of reducing experimental costs, improving accuracy, and shortening the experimental cycle

Active Publication Date: 2016-09-14
YANGZHOU UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, there is no relevant report on the specific primers for amplifying the COI gene of Lepidoptera insects in the world

Method used

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  • Specific primers for amplifying the mitochondrial coi gene of lepidopteran insects
  • Specific primers for amplifying the mitochondrial coi gene of lepidopteran insects
  • Specific primers for amplifying the mitochondrial coi gene of lepidopteran insects

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Embodiment Construction

[0016] The amplification primer screening method of the Lepidoptera COI gene of the present invention is divided into two steps: 1. Based on the sequence of the COI gene in the mitochondrial genome sequence of the Lepidoptera insect that has been measured, find out the COI gene by comparison Design primers for relatively conserved sequence fragments; 2. Amplify the COI gene of Lepidoptera insects with the synthesized primers, and test their versatility and amplification efficiency in Lepidoptera.

[0017] Below in conjunction with example the invention is further described:

[0018] 1. Sample collection: In August 2013, we collected insects from different families of Lepidoptera, mainly involving important pests in agriculture and forestry, including Noctuidae, Mothidae and Luphidae.

[0019] 2. DNA extraction: Axygen kit to extract DNA, refer to the instructions for specific steps.

[0020] 3. Data source: The data comes from the National Center for Biotechnology Information...

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Abstract

The present invention relates to a pair of primers for amplifying the gene sequence of lepidopteran mitochondrial cytochrome c oxidase I (cytochrome c oxidase I, COI), the sequences of which are shown in SEQ ID NO.1 and SEQ ID NO.2 . Using the amplification primers of the present invention can effectively amplify COI genes of various Lepidoptera species, conduct large-scale genetic background analysis on major agricultural and forestry pests in Lepidoptera, and accurately identify some close relatives that are difficult to identify Species provide an important tool for species identification, phylogeny, population genetic structure, population evolution history and geographical population identification of Lepidoptera in my country.

Description

technical field [0001] The invention relates to a pair of specific primers for amplifying mitochondrial cytochrome c oxidase I (cytochrome c oxidase I; COI) gene of lepidoptera insects. Background technique [0002] Lepidoptera has more than 40 superfamilies, more than 120 families and 180,000 species. It is the second largest order after Coleoptera, including butterflies and moths. Lepidoptera insects belong to complete metamorphosis. The development process is divided into four stages: egg, larva, pupa and adult. It is an important model organism for the study of insect mimicry, relationship with host plants, wing pattern development biology and insect physiology. [0003] The vast majority of Lepidoptera insects are agricultural and forestry pests. Smaller ones often roll leaves, attach leaves, weave silk and form nets, or drill into plant tissues to feed on them. Larger ones often eat up leaves, bite off roots, or drill branches. . On the other hand, many butterfly and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/10
Inventor 陆明星汤小天杜予州
Owner YANGZHOU UNIV
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