Bactericidal composition containing berberine and tebuconazole
A technology of tebuconazole and berberine, which is applied in the field of pesticides, can solve the problems of environmental pollution, rapid development of germ resistance, and large dosage of pesticides, and achieve the effects of reducing costs, reducing dosage, and reducing pollution
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example 1
[0029] Bioassay Example 1 : Indoor Toxicity Determination of Berberine and Tebuconazole Complex against Rice Bacterial Leaf Spot
[0030] Test object: Bacterial leaf spot of rice collected from the field
[0031] Test method: make the mother liquor with a certain concentration of the test agent, and then dilute it with water according to the set ratio to prepare 5-6 concentrations. Set clean water as the control, and add the concentration of bacteria containing 1×10 7 In the cfu / mL NB culture medium, in a constant temperature shaker at 28°C and 130r / m, after culturing for 24 hours, use a UV spectrophotometer to measure the absorbance value at a wavelength of 600nm, and calculate the antibacterial rate according to formula 2-3. The drug concentration was converted into logarithm, and the bacteriostatic rate was converted into a probability value for linear regression, and the toxicity regression equation and EC50 value (HalfMaximalEffectiveConcentration, EC50) were calculated....
Embodiment 1
[0047] Example 1 : 1% Berberine. Tebuconazole Granules
[0048]
[0049] Add xylene to berberine and tebuconazole, and then stir them fully to dissolve them completely; under stirring, slowly add propylene glycol to the system, stir fully for 30 minutes, dissolve them completely, and form a stable solution.
[0050] First add 12-60 mesh zeolite into the mixer, then add the above stable solution into it, mix for 30 minutes, after screening and testing, you can get 1% berberine. Tebuconazole granules.
Embodiment 2
[0051] Example 2 : 10% Berberine. Tebuconazole microcapsule suspension
[0052]
[0053] Dissolve 2% prothiostrobin and 8% tebuconazole in 5% xylene, then pump in 0.3% tributyl phosphate, 2.4% carboxymethyl cellulose, and 2.7% sodium alginate under high shear High-speed shear in the kettle for 30 minutes, add 20% urea-formaldehyde resin prepolymer, heat up and solidify for 60 minutes under stirring at 2000 rpm, then add deionized water, 4.5% sodium dinaphthyl methane disulfonate, 1.8% bentonite, and stir 10% berberine·tebuconazole microcapsule suspension can be obtained evenly.
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