Isaria fumosorosea oil suspending agent and preparation method as well as application thereof

A technology of I. fumigatus and oil suspending agent, which is applied in the direction of botanical equipment and methods, applications, insecticides, etc., can solve the problems of poor quick-acting performance and unstable effect, and achieve the effect of no residue in the environment

Inactive Publication Date: 2011-11-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiency of the existing I. fumigatus pesticide preparation dosage form and the deficiency of I. fumigatus in pesticide application, and provide a kind of I. fumigatus oil suspension, said oil Suspension concentrates overcome the shortcomings of poor quick-acting and unstable effects of microbial pesticides, and have the advantages of quick-acting, high activity, and good chemical and physical stability

Method used

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  • Isaria fumosorosea oil suspending agent and preparation method as well as application thereof
  • Isaria fumosorosea oil suspending agent and preparation method as well as application thereof
  • Isaria fumosorosea oil suspending agent and preparation method as well as application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The strain of I. fumigatus used in this example is derived from the house termite Coptotermes formosanus PFCF-O strain PFCF-O isolated from Shiraki-infected worms (see "Biological Characteristics of I. rosea and Its Correlation with Pathogenicity to Plutella xylostella", Acta Plant Protection Volume 37, Issue 3, June 2010, Lei Yanyuan et al.), the Insect Ecology Room of South China Agricultural University stored the strain on a PDA slant in a -80°C refrigerator. The strain grows rapidly on the PDA medium. At 25°C, the colony is long and villous, and after forming spores, it is flesh red, powdery, colorless on the back, and the pink spore bundles are solitary or aggregated into spore bundles. , the wall is smooth, transparent, mostly composed of whorled branches with 4 to 6 phialides forming whorls. The base of the bottle stem is spherical, or nearly elliptical, and the upper part is slender. The conidia are cylindrical to fusiform, smooth, transparent to light red, wi...

Embodiment 2

[0033]Transfer the commercially purchased I. fumigatus (for example, Shanghai Fuzhong Biotechnology Development Co., Ltd. or Guangzhou Institute of Microbiology) to the PDA plate, and then place it in an incubator (25 ℃, 12L:12D) for 7 days, after spore production, add rapeseed oil or plant blended oil containing 0.03% Tween 80 by mass percentage on the plate, gently scrape the spores on the plate with an inoculation needle, and the prepared Pour the bacterial solution into a beaker and stir with a magnetic stirrer for 30 minutes. After the spores are completely dispersed, drop the spore suspension onto a hemocytometer and observe with a microscope to ensure that the spore content is 1 to 10 billion / mL. If it is lower than this content, continue to add spore powder, if it is higher than this content, dilute it with the same kind of vegetable oil, observe the hemocytometer through a microscope, and make the spore content 1 to 10 billion / mL.

Embodiment 3

[0035] 1. Weigh 10.0g of 1 billion pieces / g of I. fumigatus spore suspension, peanut oil 5.0g, castor oil polyoxyethylene ether 3.0g, fatty alcohol polyoxyethylene ether 2.0, alkylphenol formaldehyde resin polyoxyethylene Vinyl ether sulfate 1.0g, epoxy linseed oil 2.0, dispersant NNO and ethylene glycol 4.0g, white mineral oil to make up to 100%, put into the grinder together, cool with water during the grinding process, the temperature should not exceed 40 ℃, 3000 revs / min and grind for 30 minutes to prepare a 10% mass percent concentration of I. fumigatus oil suspension concentrate.

[0036] 2. Indoor activity determination experiment of 10% I. fumigatus oil suspension concentrate

[0037] Test object: Pieris rapae, a sensitive strain raised indoors;

[0038] Reagent for testing: 10% Ifusaria rosea oil suspension, 10% Ifusia rosea suspension;

[0039] Conditions: Temperature: 25°C, Humidity: RH70%, Light: L:D=12:12

[0040] Test method: Accurately weigh the test agent ...

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Abstract

The invention discloses an isaria fumosorosea oil suspending agent and a preparation method as well as application thereof. The oil suspending agent is prepared from the following components in mass percent: 1-40% of isaria fumosorosea spore suspension, 1-20% of vegetable oil, 3-8% of emulsifying agent, 0.1-5% of dispersing agent, 1-5% of stabilizing agent, 1-5% of anti-freezing agent and the balance of inert mineral oil or aromatic hydrocarbon solvent. The spore of the isaria fumosorosea oil suspending agent disclosed by the invention has long survival time, and the germination rate of the spore which is stored for 2 years is 80%; the isaria fumosorosea oil suspending agent has good ageing stability and is safe to crops and environments; and the isaria fumosorosea oil suspending agent is mainly used for pest control of crops including fruit trees, vegetables, wheat, rice, flowers and the like, and especially has an obvious effect on controlling myzus persicae, whiteflies, cabbage caterpillars, cabbage moths and the like.

Description

technical field [0001] The invention belongs to the technical field of pollution-free pesticide preparations, and in particular relates to a suspension of I. fumigatus oil and its preparation method and application. Background technique [0002] Rose smoke-colored Isospora ( Isaria fumosorosea ) is an ascomycete-like entomophytic fungus, which is widely distributed all over the world and has a wide range of hosts. 25 different families of insects have been recorded, including some important agricultural pests such as diamondback moth, double-tailed aphid and whitefly. The conidia of I. fumigatus mainly invade the host by secreting a variety of extracellular hydrolytic enzymes to degrade the insect body wall. It has a mechanism of action different from that of existing chemical insecticides, and has the characteristics of no pollution to the environment and no residue. With the development of economy and society and the improvement of people's living standards, more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N63/04A01P7/04
Inventor 谢梅琼何余容吕利华王龙江
Owner SOUTH CHINA AGRI UNIV
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