Anti-Nilaparvata-lugens pesticidal protein, and coding gene and application thereof

A coding and protein technology, applied in the field of insecticidal protein against rice brown planthopper and its coding gene and application, can solve the problems of promoting the population of non-target pest insects and the degree of damage

Active Publication Date: 2015-03-04
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it also inevitably promotes the population and damage degree of non-target pest insects such as brown planthopper (Chen et al.2011)

Method used

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  • Anti-Nilaparvata-lugens pesticidal protein, and coding gene and application thereof
  • Anti-Nilaparvata-lugens pesticidal protein, and coding gene and application thereof
  • Anti-Nilaparvata-lugens pesticidal protein, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Acquisition of Novel Insecticidal Protein Gene HY151

[0062] 1. Cry1Ac and Cry54Aa1 protein and gene sequence analysis

[0063] The amino acid sequences of Cry1Ac and Cry54Aa1 were analyzed using the Blastp function of the NCBI website to analyze their conserved domains, and their corresponding nucleotide sequences were found. It is concluded that the Cry1Ac structural domain I is amino acids 36-254, and the corresponding nucleic acid coding sequence is 106-762 nucleotides; domain II is amino acids 259-461, and the corresponding nucleic acid coding sequence is 775-1383 nucleotides; domain III is amino acids 464-608, the corresponding nucleic acid coding sequence is nucleotides 1390-1824, and the rest of the sequences are linking sequences between domains, such as figure 1 shown. The Cry54Aa1 structural domain I is the 72-316th amino acid, and the corresponding nucleic acid coding sequence is the 214-948th nucleotide; the structural domain II is the 321-514...

Embodiment 2

[0111] Embodiment 2, novel insecticidal protein gene HY151 anti-insect biological activity assay

[0112] The source of tested insects: rice brown planthopper (Nilapavata lugens ) was provided by Lai Fengxiang's laboratory, China Rice Research Institute.

[0113] Samples for testing:

[0114] The anti-BPH protein HY151 prepared in Example 1 is a prokaryotic expression and purified soluble protein. The concentration and yield are shown in the corresponding protein storage tube, sent on dry ice, and stored at -80°C;

[0115] Cry54Aa1 protein: the prokaryotic expression method of Example 1 was used to prepare the Cry54Aa1 protein, and its amino acid sequence was sequence 4 in the sequence listing;

[0116] 54T protein: the prokaryotic expression method of Example 1 was used to prepare 54T protein, and its amino acid sequence was sequence 5 in the sequence listing;

[0117]Cry1Ac protein: using the method of prokaryotic expression in Example 1 to prepare Cry1Ac protein, its am...

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Abstract

The invention discloses an anti-Nilaparvata-lugens pesticidal protein, and a coding gene and application thereof. The protein is a) or b) as follows: a) protein composed of amino acid sequence disclosed as Sequence 2 in the sequence table; or b) protein with same functions subjected to substitution and / or deletion and / or addition of one or more amino acid residues on amino acid sequence disclosed as Sequence 2 in the sequence table. The experiment proves that the novel pesticidal protein disclosed by the invention has high toxicity for Nilaparvata lugens, provides a new strategy for resisting Nilaparvata lugens and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an insecticidal protein resistant to rice brown planthopper and its coding gene and application. Background technique [0002] Rice is an important food crop in the world, but rice pests and diseases seriously affect the production of rice. With the popularization and planting of Bt protein rice strains such as Cry1Ab and Cry1Ac, it is expected to effectively control the harm of Lepidoptera and Coleoptera pests (Shu, Ye et al.2000, Breitler, Vassal et al.2004, Cohen, Chen et al. al. 2008). However, it also inevitably promotes the population and damage degree of non-target pest insects such as brown planthopper (Chen et al.2011). Therefore, it is imminent to create and develop new insecticidal genes that are highly virulent to pests such as brown planthopper. [0003] Bacillus thuringiensis (Bt for short) is an Agrobacterium that forms spores and produces parasporal crystals under ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/325C12N15/32A01N47/44A01P7/04
CPCA01N63/10C07K14/325
Inventor 朱祯陈书元
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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