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Application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens

A gene and resistance technology, applied in the field of agronomy, can solve problems that threaten food security, loss, etc.

Active Publication Date: 2018-11-23
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the hidden danger of brown planthopper pests exists all the time, like a time bomb, it will burst out suddenly when the conditions are right, causing heavy losses due to too late control, directly threatening global food security

Method used

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  • Application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens
  • Application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens
  • Application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0206] Example 1 Expression profile of OsEXP10 gene

[0207] After extensive screening of the T-DNA inserted mutant library previously stored in the laboratory, a mutant with an obvious insect-susceptible phenotype was screened. After analyzing the adjacent sequence, the adjacent sequence of the mutant was obtained. Through the expression analysis of the adjacent gene, it was found that the OsEXP10 gene in the mutant was significantly overexpressed. The sequence number of the OsEXP10 gene is LOC_Os04g49410 (http: / / rice.plantbiology. msu.edu / ). In this study, the expression pattern of the gene was first analyzed, and it was found that the expression level of the gene in different tissues of the growth period of one month was relatively high, while the expression level of the gene in different tissues of the three-month-old seedlings was relatively high. low volume ( figure 1 A).

[0208] During the research on brown planthoppers in our laboratory, we noticed that the OsEXP10 g...

Embodiment 2

[0210] Example 2 OsEXP10 gene overexpression and RNAi transgenic plant phenotypes indicate that it has the function of regulating rice growth and development

[0211] The rice OsEXP10 gene was cloned, and the coding region of the gene was constructed into the overexpression plasmid p1301-35SNos to form the OsEXP10OE plasmid, which was then transformed into the wild-type rice ZH11 background by Agrobacterium-mediated genetic methods. The expression of the OsEXP10 gene in the overexpression transgenic plant was detected, and the detection result ( figure 1 B) shows that the OsEXP10 gene is significantly up-regulated in the overexpression transgenic plants.

[0212] The transgenic plants showed obvious phenotypes, which were mainly reflected in: ①The plant height of the transgenic plants became higher ( figure 2 , A, B, C), but had no significant effect on tiller number of rice ( figure 2 D); ②The grains of the transgenic plants became larger ( image 3 ). ③ Scanning electr...

Embodiment 3

[0215] Example 3 OsEXP10 gene overexpression and phenotype of RNAi transgenic plants indicate that it has the function of regulating the resistance of brown planthopper

[0216] To detect whether the OsEXP10 gene mediates the resistance to N. lugens. The overexpression of OsEXP10 gene and RNAi transgenic seedlings grown for about 50 days were identified for their resistance to BPH. The results showed that, compared with the wild-type ZH11, the transgenic plants overexpressing the OsEXP10 gene showed an obvious phenotype of susceptibility to N. lugens ( Image 6 A), while the transgenic plants of OsEXP10 gene RNAi showed obvious resistance to BPH ( Image 6 B).

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Abstract

The invention provides application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens. Specifically, the invention finds an EXP10 gene or protein thereof unexpectedly for the first time. Analysis of the expression mode of the gene finds that increase or decrease of expression quantity or activity of EXP10 gene or protein thereof in plants (such as rice) can remarkably improve agronomic character of plants for the first time.

Description

technical field [0001] The invention relates to the field of agronomy, in particular to the application of OsEXP10 gene in regulating rice growth and resistance to brown planthopper. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population depends on rice as the staple food. my country is a country with a large population, and its annual rice production and consumption account for one-third of the world's total. Therefore, the high yield and high quality of rice are directly related to the food security of my country and the world. Pests and diseases have always been one of the important factors restricting the yield and quality of rice, especially the brown plant-hopper (BPH; Nilaparvata lugens ) is a potential threat that cannot be ignored. Brown planthopper only feeds on rice and common wild rice, and is the main pest in rice production in my country and many Asian countries. In the five year...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01N57/16A01N47/44A01P7/04A01P3/00
CPCA01N47/44A01N57/16C07K14/415C12N15/8261C12N15/8282C12N15/8286A01N2300/00
Inventor 苗雪霞谭江时振英
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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