Screening method and applications of brown planthopper reference genes under high temperature stress

Abstract

The invention discloses a screening method of brown planthopper reference genes under high temperature stress. According to the screening method, fluorescent quantitative PCR studies are carried out through taking brown planthopper under no stress as a control material and brown planthoppers under the stress of different high temperatures as experimental materials; and quantitative PCR data of candidate reference genes are input to geNorm and BestKeeper softwares to be analyzed so as to screen the most suitable and most stably expressive reference gene-actin1 in the brown planthoppers under the high temperature stress and under no stress. On the basis, the expression pattern of an hsp90 gene of the brown planthopper under the high temperature stress is studied by the inventor through taking the brown planthopper at the high temperature as a sample, the heat shock protein gene hsp90 of the brown planthopper as a target gene and the screened actin1gene as the reference gene.

Description

technical field [0001] The invention belongs to the technical field of stable expression of internal reference genes in insects in molecular biology, and in particular relates to a screening method and application of internal reference genes of N. lugens under high temperature stress. Background technique [0002] With the influence of the development of modern human industry, the global climate is gradually warming. Insects are ectotherms, and the effect of temperature on insects is crucial. Excessive ambient temperature will directly seriously change the growth, reproduction, population development and survival of insects. In the past, people predicted and predicted the occurrence of pests according to environmental climate conditions, insect population base and food, etc., but high temperature conditions changed the living conditions of insects, resulting in inaccurate and biased information in insect forecast reports. Different species of insects show different levels ...

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Problems solved by technology

[0006] In recent years, researchers have discovered some genes related to heat stress response in insects, such as heat shock protein genes. Two types of hsp70 and hsp90 have also been found in brown planthopper (Genebank access numbers: JQ782193.1 and GU723300.1). However, the functions and characteristics of these genes against heat stress in N. lugens have not been studied, and the most stable internal reference genes in N. lugens under heat stress have not been reported.

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