Fluorescent reporter system for screening and identification of circular RNA translation proteins and its construction

Abstract

The invention provides a fluorescence reporting system for screening and identifying circRNA (circular ribonucleic acid) translation proteins. A nucleotide sequence of an expression cassette of the fluorescence reporting system is shown as SEQ ID NO.1. The invention further provides a construction method of the fluorescence reporting system. The fluorescence reporting system can screen and identify the circRNA translation proteins effectively.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a fluorescent reporter system for screening and identifying circular RNA translation proteins and a construction method thereof. Background technique [0002] Circular RNA (circular RNA, circRNA) is a type of RNA molecule with special functions that is different from traditional linear RNA. Most circRNAs are composed of exon sequences, which are conserved in different species and have expression specificity in tissues and different developmental stages. With the development of deep RNA sequencing and large-scale bioinformatics technology, researchers have discovered that there are a large number of circularized RNA molecules in organisms, and circularized RNAs are very stable in organisms because they form closed circles. Because circular RNA is insensitive to nucleases, it is more stable than linear RNA, which makes circular RNA have obvious advantages in the develo...

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Problems solved by technology

[0003] At present, the specific function of circular RNA has not yet been clarified, and there are only several assumptions: (1) circular RNA can act as a "sponge" (sponge) to absorb miRNA and inhibit its function; Pairing directly regulates the level of other RNAs; (3) circular RNAs can bind to proteins, inhibit protein activity, recruit components of protein complexes or regulate protein activity; some researchers have also reported that circular RNAs can also serve as templates for translation to guide proteins There are many reports on viruses and artificially synthesized circular RNA translation proteins; there are few discoveries on circular RNA translation proteins in human and other higher organisms. The reason may be that the previous research on circular RNAs was not known In addition, there is a lack of effective tools and methods for large-scale screening of circular RNA translation proteins

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