Gene clone and expression of RGD die body protein of oral gland in Japan lamprey possessing function for anti tumour

A technology of oral gland and lamprey, applied in the field of gene cloning and expression of RGD motif protein of Japanese lamprey oral gland with anti-tumor effect

Inactive Publication Date: 2006-04-19
LIAONING NORMAL UNIVERSITY
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention discovers a new gene of RGD motif protein present in the oral gland of Japanese lamprey. This gene has not been studied and reported at home and abroad. This invention is the first discovery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene clone and expression of RGD die body protein of oral gland in Japan lamprey possessing function for anti tumour
  • Gene clone and expression of RGD die body protein of oral gland in Japan lamprey possessing function for anti tumour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] 1. Extraction of total RNA: Trizol reagent of GIBCOBRL was used. details as follows:

[0063] (1) Get the venom glands of Agkistrodon halys and quickly place them in liquid nitrogen for preservation;

[0064] (2) Weigh 0.2 g venom gland tissue, add 1 ml TRIzol reagent to prepare venom gland homogenate, and incubate at 4°C for 5 min;

[0065] (3) Add 0.2ml chloroform, close the lid tightly, shake vigorously for 15sec, and then place it on ice for 5min;

[0066] (4) 4°C, 12000xg, centrifuge for 15 minutes;

[0067] (5) Transfer the upper aqueous phase to another centrifuge tube, add 0.5ml of isopropanol, and incubate on ice for 10 minutes;

[0068] (6) 4°C, 12000xg, centrifuge for 15 minutes;

[0069] (7) Discard the supernatant, add 1ml of 75% ethanol to the precipitate (containing RNA) to wash, and vortex to mix;

[0070] (8) Centrifuge at 10,000xg for 5 minutes at 4°C to obtain RNA precipitates;

[0071] (9) After air drying, dissolve with appropriate amount of T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The gene clone and expression of Japonese lampreyí»s oral gland model proteins RGD with antineoplastic action are disclosed. Three cDNA sequences with said model proteins RGD and their clones, the protein sequence and its expression in engineered bacteria, and the action of proteins RGD in closing the signal transfer channel to suppress the vascular neogenesis and tumor cell reproduction for antineoplastic purpose are also disclosed.

Description

technical field [0001] The gene cloning and expression of the Japanese lamprey oral gland RGD motif protein with antithrombotic effect belongs to the field of biotechnology, and involves the cDNA sequences and cloning of three RGD motif proteins in the Japanese lamprey oral gland, and the corresponding Protein sequence and its expression in Escherichia coli or Pichia pastoris, and the binding of these three recombinant proteins to the extracellular matrix by antagonizing the binding of certain integrins highly expressed on the surface of neovascular endothelial cells and tumor cells, blocking integrin-mediated It can inhibit angiogenesis and tumor cell proliferation, adhesion, invasion and metastasis, and cause apoptosis of vascular endothelial cells and tumor cells to achieve anti-tumor effect. Background technique [0002] The Japanese lamprey (Lampetra japonica) belongs to the class Cyclostomata, order Lamprey, family Lamprey, genus Lamprey, and is the oldest jawless vert...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/63A61K38/17A61P35/00
Inventor 李庆伟
Owner LIAONING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap