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Vesicle for treating diseases caused by helicobacter pylori

A technology of Helicobacter pylori and vesicles, applied in the field of medicine, can solve problems such as infection recurrence

Active Publication Date: 2022-01-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, H. pylori is able to escape drug treatment in the form of intracellular survival, which leads to the recurrence of infection
[0004]Therefore, the existing H. pylori eradication therapy still has great limitations

Method used

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  • Vesicle for treating diseases caused by helicobacter pylori
  • Vesicle for treating diseases caused by helicobacter pylori
  • Vesicle for treating diseases caused by helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 vesicle

[0027] Thin-film dispersion method: Weigh water-soluble or fat-soluble RHL, Chol, CLR and calcitriol, respectively, and dissolve them in chloroform for ultrasonic dispersion. Mix the dispersed RHL solution (containing 1 mg RHL), Chol solution (containing 0.5 mg Chol), CLR solution (containing 8 μg CLR) and calcitriol solution (containing 33.3 μg calcitriol) and add to the round bottom flask , after vacuum rotary evaporation, hydration and ultrasonic dispersion, and pass through a 0.22 μm filter membrane.

Embodiment 2

[0028] Example 2 Characterization of multifunctional vesicles

[0029] Encapsulation efficiency: The drug-loaded vesicles prepared in Example 1 were injected into the vesicles after methanol demulsification. Chromatographic conditions: chromatographic column is Agilent ZORBAX SB-C18 (250×4.6 mm, 5 μm); acetonitrile is used as mobile phase A, phosphate buffer is used as mobile phase B, and elution is carried out according to A:B= 48:52; flow rate The volume is 1 ml per minute; the column temperature is 45°C; the detection wavelength is 210 nm. The encapsulation efficiency was calculated according to the following formula.

[0030] Encapsulation rate (%) =

[0031] in, W1 is the drug content encapsulated in the vesicles, W0 is the amount of drug added to the system.

[0032]Experimental results: the drug encapsulation efficiency of vesicles made of water soluble RHL (water soluble-RHL vesicle, WRV) and liposoluble RHL vesicles (liposoluble-RHL vesicle, LRV) are shown in T...

Embodiment 3

[0038] Example 3 The scavenging effect of vesicles on bacterial film

[0039] (1) Dilute with Brain Heart Infusion Broth (BHI) containing 2% FBS H. pylori bacterial suspension to OD 600nm The value is 0.2, and inoculated in a 48-well plate, and put into a microaerophilic gas bag of the corresponding specification to maintain a suitable gas environment (5% O 2 , 85% N 2 , 10%CO 2 ), and cultured in a 37°C incubator for 72 h. (2) Drug treatment: ① In order to explore the influence of the main components in the vesicles on the removal of bacterial film, the present invention prepared vesicles with different contents of Chol and CLR while keeping the content of RHL unchanged. The drug-loaded vesicles were diluted so that the highest concentration of vesicles in each group was consistent, all containing 600 μg / ml RHL, and diluted according to the double method. Take the cultured pellicle out of the airtight jar and wash it with sterile PBS to remove free H. pylori Afterward...

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Abstract

The invention relates to a vesicle for treating diseases caused by Helicobacter pylori infection, wherein the vesicle comprises: a lipid bilayer constituting the main body of the vesicle; cholesterol and helicobacter pylori sensitive antibiotics which are embedded in the lipid bilayer and are used for repairing a host cell lipid raft structure to block immune escape; and caltriol which is used for recovering the degradation effect of autolysosome to kill intracellular helicobacter pylori; wherein the lipid is phospholipid or / and rhamnolipid. The invention also relates to a pharmaceutical composition which comprises the vesicle for treating diseases caused by helicobacter pylori infection and a pharmaceutically acceptable carrier. According to the vesicle disclosed by the invention, the removal rate of helicobacter pylori is further improved by recovering immune response and enhancing the killing effect of intracellular bacteria on the basis of removing pellicle, killing free bacteria and inhibiting readhesion of residual bacteria.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a vesicle used for treating diseases caused by Helicobacter pylori. Background technique [0002] Helicobacter pylori ( Helicobacter pylori , H. pylori ) is a screw-shaped, microaerophilic, Gram-negative bacterium that has been found to colonize the luminal surface of gastric epithelial cells. It has been reported that, H. pylori Infection is a disease state in which all H. pylori All infected persons should be treated for eradication. However, large doses and long-term use of antibiotics can induce the emergence of drug-resistant strains, leading to H. pylori Treatment faces more serious challenges. [0003] H. pylori Susceptible to many antibiotics in vitro, but most antibiotics are not resistant to gastric acid environment. In addition, the presence of gastric mucosa also weakens the delivery effect of antibiotics. eradicate H. pylori Failure is related to a...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/26A61K45/06A61K31/575A61K31/593A61K31/43A61K31/7048A61P31/04A61P35/00A61P1/04
CPCA61K9/1272A61K47/26A61K45/06A61K31/575A61K31/593A61K31/43A61K31/7048A61P31/04A61P35/00A61P1/04A61K2300/00
Inventor 胡海燕陈小楠邹祎晴李彭宇饶义琴
Owner SUN YAT SEN UNIV
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