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Dual-channel structure lighting super resolution imaging method and device

A structured light illumination, dual-channel technology, applied in optics, optical components, microscopes, etc., can solve the problem of diffraction limit of image spatial resolution

Active Publication Date: 2015-11-18
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
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Problems solved by technology

Currently, such probes are limited to detection on traditional dual-channel fluorescence microscopes, and the spatial resolution of the obtained images is limited by the diffraction limit.

Method used

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  • Dual-channel structure lighting super resolution imaging method and device
  • Dual-channel structure lighting super resolution imaging method and device
  • Dual-channel structure lighting super resolution imaging method and device

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Embodiment Construction

[0042] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0043] See attached figure 1 The dual-channel structured light illumination super-resolution microscopic imaging system provided in Embodiment 1 of the present invention includes an ordinary fluorescence microscope 11, and the ordinary fluorescence microscope 11 includes a fifth lens 12, a first dichromatic mirror 13, an objective lens 14, and a sample stage arranged in sequence 15. The VI lens 16. The imaging system also includes a light source 1, a first reflecting mirror 2, a second reflecting mirror 3, a first lens 4, a diaphragm 5, a second lens 6, a spatial light modulator 7, a third lens 8, a light baffle 9, a first IV lens 10, II dichromatic mirror 17, I band-pass filter 18, II band-pass filter 19, I detector 20 and II detector 21. The incident angle of the laser light incident on the spatial light modulator 7 is less than 1...

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Abstract

The present invention discloses a dual-channel structure lighting super resolution imaging method and a device. The device comprises an ordinary fluorescent microscope, a light source, a first mirror, a second mirror, a first lens, an aperture, a second lens, a spatial light modulator, a third lens, a light barrier, a fourth lens, a second dichroic mirror, a first band-pass filter, a second band-pass filter, a first detector and a second detector. Meanwhile, the present invention also discloses a microscopic imaging method based on the imaging system. According to the imaging system and the imaging method, the heterogeneity of lipid rafts on a cell membrane can be measured in a hyperspace resolution scale.

Description

technical field [0001] The invention relates to a dual-channel structured light illumination super-resolution imaging method and device. Background technique [0002] Lipid rafts play an important role in signal transduction on the plasma membrane, and it is of great significance to directly observe the microstructure of lipid rafts and their corresponding cellular functions by using fluorescence microscopy. Lipid rafts cannot be detected due to the diffraction-limited resolution of conventional fluorescence microscopy. Some super-resolution imaging techniques developed in recent years, such as stimulated radiation depletion microscopy (STED) and photoactivated localization microscopy (PALM), have also recently been used in the study of lipid rafts. However, they have only been used for Probes clusters of lipid rafts without being able to detect the physical environment surrounding the rafts. Some recently developed environment-sensitive probes can reflect the physical env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00G02B21/06G02B27/58
Inventor 陈建玲杨洪钦邱彩敏谢树森王瑜华郑莉琴
Owner FUJIAN NORMAL UNIV
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