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Protein chip for detecting multiple veterinary drug residues and kit thereof

A technology for protein chips and veterinary drug residues, applied in measuring devices, material analysis by observing the impact on chemical indicators, instruments, etc., can solve problems such as incomplete controllability, improve efficiency, ensure safety and sanitation, and avoid potential Hazard and Pollution Effects

Inactive Publication Date: 2010-07-21
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although joint detection can be achieved in this way, the detection of the same sample needs to be carried out in different coated carrier tanks, the consistency of the reaction conditions of multiple different index carrier tanks, whether there is contamination by endogenous and exogenous substances, and quality control, etc. , are not fully controllable

Method used

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  • Protein chip for detecting multiple veterinary drug residues and kit thereof
  • Protein chip for detecting multiple veterinary drug residues and kit thereof
  • Protein chip for detecting multiple veterinary drug residues and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of a protein chip for detecting residues of various veterinary drugs

[0037] The main steps are: (1) Prepare the matrix nitrocellulose membrane; (2) Design the chip, determine the arrangement of the lattice and the spotting position; (3) Draw the antibody from the 384-well plate and spray it on the nitrocellulose (4) assemble a nitrocellulose membrane, and carry out sealing, drying, packaging, and preservation.

[0038] The above preparation method for detecting multiple veterinary drug residue detection protein chips specifically includes the following steps:

[0039] 1. Determination of veterinary drug detection antigen antibody

[0040] Determination of detection index antigen:

[0041] Eight kinds of veterinary drugs that are more harmful and more widespread were selected as antigens for the preparation of spotting antibodies. Antigens were purchased from the market.

[0042] Determination of detection index spotted antibody:

[0043] The ...

Embodiment 2

[0055] Embodiment 2: the preparation of kit

[0056] A kit for detecting protein chips for residues of various veterinary drugs, which is composed of protein chips for detecting residues of various veterinary drugs, sample diluent, enzyme label working solution, concentrated washing solution, detection solution, positive control, and negative control; each part is bottled, Fix it in the box with a paper template (such as figure 2 ), not limited to the placement method, it is used together with the test. Here's how the parts are prepared:

[0057] 1. The protein chip for the detection of various veterinary drug residues is a 6×8 well plate, with nitrocellulose membrane as the base, and the assembly method is shown in ZL032298900. After the completion of the appearance, see figure 1 .

[0058] 2. Provide 1 bottle of negative control 5, 0.25ml.

[0059] Positive control 6 provides 3 bottles, 0.25ml in each bottle.

[0060] Prepare with phosphate buffer (pH 7.4) containing ...

Embodiment 3

[0071] Example 3: Application and detection of protein chips and kits

[0072] The application of the protein chip for quantitative detection of various veterinary drugs according to the present invention mainly uses the method of antibody-antigen-enzyme-labeled secondary antibody to detect veterinary drugs in samples. The method is: take 1g of the sample and mash it, add 1ml of the sample diluent 3, mix well, let the sample fully react with the sample diluent 3 for half an hour, absorb part of the extract, and add it to the surface of the chip. The veterinary drug present in the sample extract will be used as the detection antigen and react with the corresponding specific antibody immobilized on the chip; absorb the reaction solution, add the enzyme label working solution 7 to react, and then wash away the unbound enzyme label 2 Anti-resistance; after adding an appropriate amount of detection solution, react properly, scan and collect signals, and the signal intensity of the ...

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Abstract

The invention discloses a protein chip for detecting multiple veterinary drug residues and a kit thereof. The protein chip comprises a substrate and dot coatings of veterinary drug antibody, wherein the dot coatings are distributed in an array type, the substrate is a glass substrate or a membrane substrate; and the dot coatings of the veterinary drug antibody refer to eight types of veterinary drug antibodies, i.e. an anti-chloramphenicol antibody, an anti-chlorpromazine antibody, an anti-colchicin antibody, an anti-dapsone antibody, an anti-stilboestrol antibody, an anti-dimetridazole antibody, an anti-furazolidone antibody and an anti-metronidazole antibody which are uniformly distributed and in dot lattice on the substrate. The protein chip can obtain the reaction results with multiple indexes by only one-step reaction and can be suitable for simultaneously detecting eight veterinary drugs, i.e. chloramphenicol, chlorpromazine, colchicin, dapsone, stilboestrol, dimetridazole, furazolidone and metronidazole which are contained in a sample, thereby ensuring the safety and the sanitation of a large quantity of meat products.

Description

technical field [0001] The invention relates to veterinary drug detection products, in particular to a protein chip for detection of multiple veterinary drug residues and a kit thereof. Background technique [0002] my country is a big country in the production and consumption of agricultural products. With the modernization, intensification and large-scale production of animal husbandry, veterinary drugs and veterinary drug additives play an important role in reducing morbidity and mortality, improving feed utilization, promoting growth and improving meat quality. Significant role, has become an indispensable material basis for modern animal husbandry. However, due to the lack of scientific knowledge and the drive of economic interests, the abuse of veterinary drugs and the excessive use of veterinary drugs in the breeding process are common. Its consequences, on the one hand, lead to veterinary drug residues in animal-derived foods, which affect human health after ingestio...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N21/78
Inventor 穆海东汪宁梅穆宇豪奚鹰刘纲
Owner 上海裕隆生物科技有限公司
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