Application and preparation method of dual-functional diethylstilbestrol non-enzymatic sensor constructed on basis of two-dimensional composite material

A technology of diethylstilbestrol and composite material, which is applied in the fields of new nano-functional materials and electrochemical biosensing analysis, can solve the problems of limiting wide application and affecting the convenience of operation, etc., and achieves highly selective detection and convenient operation. , The effect of improving detection sensitivity and detection limit

Inactive Publication Date: 2016-07-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since the electrochemiluminescence sensor requires external light signal capture devices such as photodiodes, and the photoelectrochemical sensor requires an external light

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 A bifunctional diethylstilbestrol enzyme-free sensor based on a two-dimensional composite material. The specific preparation steps are:

[0039] (1) Zn-MoS 2 Preparation of:

[0040] 0.005g zinc chloride ZnCl 2 , 2mL of 0.1mol / L ascorbic acid solution, 0.5mL of sodium molybdate Na 2 MoO 4 solution and 0.01 g sodium sulfide Na 2 S, after mixing and stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain Zn-MoS 2 , which was dissolved in deionized water to obtain Zn-MoS 2 solution.

[0041] (2) Preparation of ECL working electrode W1:

[0042] 1) Using ITO conductive glass as the working electrode, drop-coat the Zn-MoS prepared in (1) on the surface of the electrode 2 Solution, covering an area of ​​1cm × 1cm, dry at room temperature;

[0043] 2) Put the working electrode obtained in 1) on the Zn-MoS 2 The ...

Embodiment 2

[0060] Example 2 A bifunctional diethylstilbestrol enzyme-free sensor based on a two-dimensional composite material. The specific preparation steps are:

[0061] (1) Zn-MoS 2 Preparation of:

[0062] The preparation steps are the same as that of Zn-MoS in Example 1 2 The preparation method of the solution, the difference is: ZnCl 2 The addition amount of ascorbic acid solution is 0.008g, the addition amount of ascorbic acid solution is 4mL, Na 2 MoO 4 The amount of solution added was 1.0 mL and Na 2 The amount of S added was 0.02 g, and the reaction was carried out at 220° C. for 12 hours.

[0063] (2) Preparation of ECL working electrode W1:

[0064] The preparation steps are the same as those of W1 in Example 1, except that the Zn-MoS prepared in (1) in this example is used 2 Modified electrodes; the length of the gold nanorods is 30nm; the concentration of luminol in the electrolyte is 5mmol / L, the concentration of sulfuric acid is 0.5mol / L, and the electrochemical...

Embodiment 3

[0072] Example 3 A bifunctional diethylstilbestrol enzyme-free sensor based on a two-dimensional composite material. The specific preparation steps are:

[0073] (1) Zn-MoS 2 Preparation of:

[0074] The preparation steps are the same as that of Zn-MoS in Example 1 2 The preparation method of the solution, the difference is: ZnCl 2 The addition amount of ascorbic acid solution is 0.01g, the addition amount of ascorbic acid solution is 6mL, Na 2 MoO 4 The amount of solution added was 1.5mL and Na 2 The amount of S added was 0.03 g, and the reaction was carried out at 220° C. for 12 hours.

[0075] (2) Preparation of ECL working electrode W1:

[0076] The preparation steps are the same as those of W1 in Example 1, except that the Zn-MoS prepared in (1) in this example is used 2 Modified electrodes; the length of the gold nanorods is 40nm; the concentration of luminol in the electrolyte is 10mmol / L, the concentration of sulfuric acid is 1.0mol / L, and the electrochemical ...

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Abstract

The invention discloses a preparation method of a functional nanomaterial based biological immunosensor with dual signal sending strategies of electrochemiluminescence and photoelectrochemistry.The sensor prepared according to the method has the advantages of simplicity in operation, portability, quickness in detection and low in cost and can be used for rapid and sensitive detection of diethylstilbestrol in fields of daily production, daily life and the like.

Description

technical field [0001] The invention relates to a preparation method of a sensor for detecting diethylstilbestrol, and the sensor integrates dual functions of electrochemiluminescence and photoelectrochemistry. It belongs to the field of new nano functional materials and electrochemical biosensing analysis technology. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Diethylstilbestrol is a synthetic non-steroidal estrogen substance. At present, the methods for detecting diethylstilbestrol mainly include chromatography and mass spec...

Claims

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Application Information

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IPC IPC(8): G01N21/76B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00G01N21/76
Inventor 张勇魏琴杜斌任祥胡丽华
Owner UNIV OF JINAN
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