Multifunctional feed special for wood frog and polliwog
A multifunctional, tadpole technology, applied in animal feed, animal feed, applications, etc., can solve the problems of farmers' economic losses, environmental forest frog product pollution, and incomplete nutrition, so as to improve disease resistance and nutrient absorption capacity, Effects of preventing forest frog diseases and improving immunity
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Embodiment 1
[0043] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.
[0044] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,
[0045] The composi...
Embodiment 2
[0061] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.
[0062] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,
[0063] The composi...
Embodiment 3
[0079] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.
[0080] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,
[0081] The composi...
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