Multifunctional feed special for wood frog and polliwog

A multifunctional, tadpole technology, applied in animal feed, animal feed, applications, etc., can solve the problems of farmers' economic losses, environmental forest frog product pollution, and incomplete nutrition, so as to improve disease resistance and nutrient absorption capacity, Effects of preventing forest frog diseases and improving immunity

Inactive Publication Date: 2008-12-31
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the feed used for tadpoles is mainly simple feed such as corn paste prepared by the farmers themselves. The nutrition is not comprehensive, so that the tadpoles grow slowly, are prone to diseases, directly affect the metamorphosis of the tadpoles, and the economic benefits are not high; secondly, the hydrophilic aeromonas Bacteria are pathogenic microorganisms widely present in aquatic organisms, and can produce highly toxic exotoxins, such as: hemolysin, histotoxin, necrotic toxin, enterotoxin and protease, etc. After Aeromonas hydrophila invades the body, it will first Proliferate in the tract, and then enter the liver, kidney and other tissues through the portal artery circulation, causing liver, kidney and other organs and blood lesions, and then systemic symptoms, which can cause red leg disease, hemorrhagic disease and other diseases in wood frogs and tadpoles, and the mortality rate is extremely high High, causing huge economic losses to farmers
Furthermore, the male to female ratio of hatched wood frogs in the natural state is between 3:7 and 4:6, that is, there are fewer females, and the price of females is more than 10 times that of males, thus limiting the economic benefits of farmers
In addition, with the increase in the price of forest frogs, in order to improve the survival rate, farmers use a large number of drugs such as antibiotics to control the disease of forest frogs, thus causing certain pollution to the environment and forest frog products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.

[0044] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,

[0045] The composi...

Embodiment 2

[0061] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.

[0062] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,

[0063] The composi...

Embodiment 3

[0079] (1) Preparation of inactivated Aeromonas hydrophila vaccine: use an inoculation needle to take Aeromonas hydrophila stored in glycerol cryopreservation solution, smear it on an LB plate, streak it for recovery, and pick a single vaccine after culturing overnight at 28°C. Bacterial colonies were inoculated in 1L liquid LB seed medium at 28°C and 200rpm on a shaker until OD600=3.0, then inoculated in a fermenter for culture (28°C, 700rpm, dissolved oxygen 60%), and the bacteria were harvested when OD600=3.0, 8000 rpm Centrifuge for 10 minutes, and adjust the cell concentration to 100 g / L with 1% formaldehyde to inactivate for 48 hours. After the sterility test confirms that there is no live bacteria, add sodium alginate and stir to coat the bacteria, and make the Aeromonas hydrophila inactivated microcapsule vaccine for future use.

[0080] (2) the special feed for multifunctional Rana tadpoles, consisting of main ingredients and auxiliary materials,

[0081] The composi...

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PUM

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Abstract

The invention relates to a multifunctional special feed for Tadpole of Rants chensinensis. The technical proposal adopted is that the feed consists of main materials and auxiliary materials; wherein, every ton of the main materials consists of 200 to 400kg of rice bran, 100 to 200kg of corn meal, 100 to 200kg of fish meal, 100 to 300kg of bean pulp powder, 100 to 200kg of meat meal, 50 to 100kg of wheat-middling and 20 to 70kg of wheat bran; every ton of the main materiala is added into the following auxiliary materials by weight: 200 to 800g of microbial inoculum agent, 200 to 500g of aquaculture vitamin complex, 200 to 500g of aquaculture compound mineral, 200 to 500g of antioxidant, 20 to 100g of inactivated aeromonas hydrophila vaccine and 20 to 50g of diethylstilbestrol. The preparation method is that firstly, the main materials are smashed, stirred; and then the auxiliary materials are added into the main materials and are stirred evenly, regulated in steam, granulated and cooled. The feed has the functions of sex induction and disease prevention for the Tadpole of Rants chensinensis.

Description

Technical field: [0001] The invention relates to the field of feed, in particular to a feed with the sex-inducing function of tadpoles. Background technique: [0002] Chinese forest frog, commonly known as "hashi toad", is an important medicinal amphibian economic animal. It is widely distributed in the Changbai Mountains and Xiaoxing'an Mountains in Northeast China. The dried oviduct product of the female frog is the famous "hashi toad oil". , is a precious dietary Chinese medicine, containing a variety of saturated fatty acids, amino acids, vitamins and trace elements, with the effects of nourishing yin and lungs, tonifying kidney and essence, strengthening the brain and anti-fatigue. With the improvement of people's living standards, the demand for Rana is also increasing, so the artificial breeding of Rana is also developing rapidly. At present, the cultivation of Rana is mainly semi-artificial breeding, that is, it mainly manages the tadpole stage. At present, the feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/175A23K1/00A23K10/20A23K10/22A23K10/30A23K10/37A23K20/20A23K50/80
CPCY02A40/818Y02P60/87
Inventor 王秋雨金莉莉李强宋树森
Owner LIAONING UNIVERSITY
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