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Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same

A technology of estriol and estradiol, which is applied in the field of estrogen veterinary drug residue detection, can solve the problems of high professional requirements and cumbersome operation, and achieve the effects of easy preparation, accurate results and saving detection costs

Inactive Publication Date: 2011-05-04
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a colloidal gold test paper that is easy and quick to operate, low in cost, easy to carry, can be operated by non-professionals, and can be detected on-site, and can detect three types of estrogens at the same time, overcoming the cumbersome operation of the prior art. High professional requirements and defects that can only be detected one by one

Method used

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  • Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same
  • Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same
  • Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same

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Experimental program
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Effect test

Embodiment 1

[0023] A triple detection card for estradiol, estriol and diethylstilbestrol, which is provided with a test strip 2 in the detection card casing 1, the structure of the detection card casing 1 and the setting of the test strip 2 refer to the attached figure 1 , figure 2 . The detection card shell 1 is a long flat shell-shaped shell, 7cm long, 2cm wide, 0.5cm thick, and the shell wall thickness is 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0024] The test strip 2 is a thin strip with a multilayer structure, the length of the strip is about 6.0cm, the width of the strip is about 0.4cm, and the thickness is less than 2.5mm. The test strip 2 has a multilayer structure: the bottom layer is a supporting ...

Embodiment 2

[0027] The processing method of the test sample:

[0028] The treatment method for urine samples is: the urine is directly used as a test sample. If the urine sample is turbid, it needs to be filtered through filter paper first, and the supernatant is taken as a test sample; if the urine is clear, it is directly used as a test sample.

[0029] The treatment method for feed samples is: weigh 0.5 g of feed samples, add 1 mL of sample extract (pH 7.0, 50 mmol / L Tris-HCL), stir in the test tube for 1 minute, and after standing for 10 minutes, take the clear upper Serum was used as test sample.

[0030] The processing method for animal tissue, meat, and liver samples is: take 1 gram of shredded or homogenized animal tissue, meat, and liver samples, and place them in 1 mL of sample extract (Tris-HCL pH 7.0, 50 mmol / L ) in a test tube, tighten the cap tightly, shake the sample for one minute, put it in a water bath at 100°C for 5 minutes, cool to room temperature, and take the clear...

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Abstract

The invention relates to an estradiol, estriol and diethylstilbestrol three-joint detection card and a processing method of a sample detected by the same, belonging to the technical field of residue detection of estrogen veterinary drugs. A test strip is arranged in a housing of the three-joint detection card, a sample pad, a colloidal gold film, a nitrocellulose film and a water absorbing film are sequentially pasted on a supporting back plate, wherein the colloidal gold film is a glass fiber film containing an estradiol antibody, an estriol antibody and a diethylstilbestrol antibody colloidal gold marker, three detection strips are arranged on the nitrocellulose film and respectively comprise an estradiol protein coupling matter, an estriol protein coupling matter and a diethylstilbestrol protein coupling matter, and in addition, a quality controlling belt containing a rabbit antibody or mat antibody is arranged on the nitrocellulose film. The estradiol, estriol and diethylstilbestrol three-joint detection card can simultaneously detect estradiol, estriol and diethylstilbestrol contained in urine or feeds as well as animal tissues, meat and livers, and has the advantages of easiness to preparation, detection expense saving, convenience and quickness in use and accurate result.

Description

[0001] technical field [0002] The invention relates to a device for detecting estrogen veterinary drugs, in particular to a triple detection card of estradiol, estriol and diethylstilbestrol and a processing method for detection samples thereof, belonging to the technical field of detection of estrogen veterinary drug residues. Background technique [0003] It is less than ten years since human beings have generally recognized and systematically studied the environmental pollution and harm caused by animal endocrine disruptors, but more and more studies have shown that the continuous accumulation of endocrine disrupting pollutants caused Pollution is causing harm to humans and wildlife in various ways, and it has been widely detected in natural water bodies, urban sewage treatment systems and water supply systems worldwide. [0004] Among them, the pollution of estrogen is more serious. The most widely used natural hormones are estradiol, estriol and artificially synthesiz...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N1/28G01N1/34
Inventor 周坚熊晓芳吴文晔
Owner 无锡安迪生物工程有限公司
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