Glassy carbon electrode modified electrochemical detection method for diethylstilbestrol

A glassy carbon electrode, diethylstilbestrol technology, applied in the field of electrochemical analysis, can solve the problems of high detection limit, unable to meet the minimum detection limit of hexene residues, poor repeatability, etc. Human, reproducible results

Inactive Publication Date: 2007-10-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of this method are: due to the use of carbon paste electrodes, its repeatability is not good, and the detection limit

Method used

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  • Glassy carbon electrode modified electrochemical detection method for diethylstilbestrol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment 1 is implemented under the following conditions of implementation and technical requirements:

[0038] (1) Preparation of polyethyleneimine-nanogold modified electrode

[0039] (1) Electrode pretreatment:

[0040] First, the glassy carbon electrode is used on the suede with TiO 2 The nano-powder is polished in the shape of "8" to make it into a mirror surface, rinsed with distilled water, and then cleaned with ultrasonic waves for 10 minutes. Then put the cleaned glassy carbon electrode into nitric acid solution and acetone solution with a volume ratio of 1:1 and soak for 3 minutes respectively, and rinse the electrode with distilled water. Finally, the electrodes were dried under infrared lamps.

[0041] (2) Preparation of nano-gold colloidal particles: first add 0.5 ml of 1% chloroauric acid solution to a large beaker with 200 ml of distilled water. Then place the beaker on the electric stove and bring to a boil over high heat. Use a pipette gun t...

Embodiment 2

[0056] This embodiment 2 is implemented under the following conditions of implementation and technical requirements:

[0057] (1) Preparation of polyethyleneimine-nanogold modified electrode

[0058] (1) Electrode pretreatment:

[0059] Firstly, the process of grinding the glassy carbon electrode on the suede and rinsing with distilled water is the same as in Example 1. Then ultrasonic cleaning was used for 30 minutes, and then the cleaned glassy carbon electrode was put into nitric acid and acetone solutions with a volume ratio of 1:1 and soaked for 15 minutes respectively, and the electrode was rinsed with distilled water. Finally, the electrode was dried naturally in the air.

[0060] (2) Preparation of nano-gold colloidal particles: first add 1.0 ml of 1% chloroauric acid solution into a large beaker with 200 ml of distilled water. Then place the beaker on the electric stove and bring to a boil over high heat. Use a pipette gun to add 2.0ml of freshly prepared 1% triso...

Embodiment 3

[0070] This embodiment 3 is implemented under the following conditions of implementation and technical requirements:

[0071] (1) Preparation of polyethyleneimine-nanogold modified electrode

[0072] (1) Electrode pretreatment:

[0073] Firstly, the process of grinding the glassy carbon electrode on the suede and rinsing with distilled water is the same as in Example 1. Then ultrasonic cleaning was used for 20 minutes, and then the cleaned glassy carbon electrode was put into nitric acid and acetone solutions with a volume ratio of 1:1 and soaked for 9 minutes respectively, and the electrode was rinsed with distilled water. Finally, the electrodes were dried naturally in the air.

[0074] (2) Preparation of nano-gold colloidal particles: first add 0.75ml of 1% chloroauric acid solution into a large beaker with 200ml of distilled water. Then place the beaker on the electric stove and bring to a boil over high heat. Use a pipette gun to add 1.0ml of freshly prepared 1% triso...

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Abstract

The invention relates to an electrochemical check method of glass-carbon electrode decoration of estromon, belonging to electrochemical analysis technical field, comprising that electrode pretreatment, nanometer gold sol preparation, polyethylene imine-nanometer gold decorated glass carbon electrode preparation, more particularly comprising that preparing polyethylene imine decorated glass-carbon electrode and preparaing polyethylene imine nanometer gold decorated electrode, and using the polyethylene imine nanometer gold decorated electrode to test the content of estromon. The inventive decoration of the working electrode is simple and repeatable, while the decorated electrode has strong selective collection on estromon, better test repeatability and high sensitivity, while the check limit can reach 0. 001mug/g.

Description

technical field [0001] The invention relates to a detection method in the technical field of electrochemical analysis, in particular to an electrochemical detection method modified by a glassy carbon electrode of diethylstilbestrol. Background technique [0002] Diethylstilbestrol ((E)-3,4-Bis(4-hydroxyphenyl)-3-hexene, DES) was born in the 1940s and is the first orally active synthetic estrogen. Diethylstilbestrol is one of the most potent artificial estrogens. It is mainly used in medicine to treat estrogen deficiency, but because of its effects of promoting animal growth, increasing protein deposition and improving feed conversion rate, it was once widely used as a growth promoter in animal husbandry. In veterinary clinical practice, diethylstilbestrol is mainly used for aphrodisiac in animals that are not obviously in estrus, and it can also be used to treat retained placenta, expel stillbirth, and treat metritis. Since it was discovered that the residues of DES-contai...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/413G01N27/38G01N33/74
Inventor 刘国艳柴春彦
Owner SHANGHAI JIAO TONG UNIV
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