Diethylstilbestrol enzyme linked immunoassay reagent box and determination

A diethylstilbestrol enzyme and immunological detection technology, which is applied in biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2003-11-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The determination and instrumental analysis methods of diethylstilbestrol and some other steroid hormones in beef an...

Method used

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  • Diethylstilbestrol enzyme linked immunoassay reagent box and determination
  • Diethylstilbestrol enzyme linked immunoassay reagent box and determination
  • Diethylstilbestrol enzyme linked immunoassay reagent box and determination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] 1. Carry out sample processing according to the above method.

[0098] 2. Reagent preparation

[0099] Diethylstilbestrol antibody dilution solution, (Diethylstilbestrol antibody concentrate: dilution = 1:10),

[0100] Diethylstilbestrol peroxidase marker dilution solution, (enzyme marker concentrate: dilution = 1:10).

[0101] 3. Add antibody and incubate at room temperature: add 100ul diluted antibody solution to the bottom of each microwell. Incubate for 15 minutes at room temperature.

[0102] 4. Washing: Pour out the liquid in the well, turn the microwell rack upside down on the absorbent paper and pat (3 times per row) to ensure that the liquid in the well is completely removed. Fill the wells with 250ul distilled water, pour out the liquid in the microwells again, and repeat the operation twice.

[0103] 5. Add standard solution or sample to be tested: Add 20ul of standard or processed sample to the respective microwells, and do two parallel experiments for s...

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Abstract

A chemical reagent box applies enzyme linked immunosorbent assay method to detect diethylstilbestrol. The cony IgG sensitizer is enveloped on the enzyme scale plate and the two are reacted through hatching, washnig, sample diluent and enzyme scale antigen adding after diethyl stilbestrol sensitizer is added. The detection is finished by washing, adding enzyme-substrate material to display coloure and using enzyme scale meter to detect A 450 nm.

Description

technical field [0001] An enzyme-linked immunoassay kit for diethylstilbestrol and a detection method thereof are used for detecting the content of diethylstilbestrol in animal foods. The invention belongs to the technical field of enzyme-linked immunoassay. Background technique [0002] Diethylstilbestrol (Diethylstilbestrol) is a synthetic hormone drug that can be applied clinically to the treatment of menopausal syndrome. In recent years, "food tonic" health products have gradually become a trend to replace medicines, and more and more people accept them. Some medicines are added to the feed of poultry and livestock in order to contain these trace medicines in their eggs and meat, so as to achieve Replace medicine with food. [0003] The determination and instrumental analysis methods of diethylstilbestrol and some other steroid hormones in beef and animal blood have been reported in the literature, but the determination of diethylstilbestrol in eggs is rarely reported....

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N33/53G01N33/535G01N33/573G01N33/74
Inventor 胥传来李相前王武康邵蔚蓝徐保国贺铁明胡拥明
Owner JIANGNAN UNIV
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