Aspergillus niger for effectively degrading diethylstibestrol

A technology of diethylstilbestrol and Aspergillus niger, which is applied in the field of environmental treatment by microorganisms, and achieves the effects of fast degradation, low cost and simple operation steps

Inactive Publication Date: 2015-08-12
SICHUAN AGRI UNIV
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At present, there is no report about the fungus with the abili

Method used

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  • Aspergillus niger for effectively degrading diethylstibestrol
  • Aspergillus niger for effectively degrading diethylstibestrol
  • Aspergillus niger for effectively degrading diethylstibestrol

Examples

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Effect test

example 1

[0015] Example 1: Degradation of diethylstilbestrol by strain YAT at different culture times.

[0016] Seed solution preparation: pick YAT strains and streak on PDA plate, culture in 28 °C constant temperature incubator for 72 h, pick single colony and streak on PDA slant medium, culture in 28 °C constant temperature incubator for 72 h h; the YAT bacterial lawn on the slant culture medium was eluted with sterile physiological saline and mixed evenly to make seed liquid (spore concentration was about 10 8 CFU / mL).

[0017] Take 1 mL of seed liquid and inoculate it in 29 mL (250 mL Erlenmeyer flask) of MM medium containing 0.2% Tween 80 with a diethylstilbestrol concentration of 20 mg / L, and replace the seed liquid with 1 mL of normal saline as the blank control, and store at 28 °C , 180 r / min shaking culture for 4 days, sampling every 24 hours, methanol ultrasonic extraction, HPLC detection of diethylstilbestrol concentration. The HPLC spectra of diethylstilbestrol before an...

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Abstract

The invention relates to the field of environment treatment using microbes, and concretely relates to an Aspergillus niger for metabolizing diethylstilbestrol. The Aspergillus niger for metabolizing diethylstilbestrol is Aspergillus niger YAT, and the Aspergillus niger YAT is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on Feb. 11, 2015 with the preservation number of CGMCC No.10568. The Aspergillus niger can effectively degrade 20mg/L of diethylstilbestrol, and can be processed to develop corresponding environmentally friendly biological preparations, provides important resources for degradation or removal of diethylstibestrol residual in environment and foods, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of environmental treatment by using microorganisms, in particular to a strain of Aspergillus niger capable of degrading diethylstilbestrol. Background technique [0002] Diethylstilbestrol is a synthetic non-steroidal estrogen drug that has been used as a drug to improve the reproductive performance and production performance of animals. As an endocrine disruptor, diethylstilbestrol can cause liver damage, gene mutation, hormone imbalance in human body, female breast cancer, ovarian cancer and other diseases. As an endocrine disruptor, diethylstilbestrol has received extensive attention from the scientific community and the public. Due to its impact on the hormone system and its chronic toxicity to the body, it was banned by the US FDA in 1970. The "2003 Veterinary Drug Residue Monitoring Plan in Animal Products" issued by the Ministry of Agriculture of the People's Republic of China requires that diethylstilbestrol...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02C02F3/34C12R1/685A62D101/28C02F101/38
CPCB09C1/105C02F3/34C02F2101/345C12N1/145C12R2001/685
Inventor 刘书亮胡凯弟文涛胡露罗松明胡欣洁陈姝娟
Owner SICHUAN AGRI UNIV
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