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Aspergillus oryzae capable of effectively degrading diethylstilbestrol

A technology of diethylstilbestrol and Aspergillus oryzae, applied in the field of a strain of Aspergillus oryzae that effectively degrades diethylstilbestrol, achieving the effect of simple operation steps, low cost, and safe bacterial source

Inactive Publication Date: 2016-07-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the fungi or food-derived strains that have the ability to degrade diethylstilbestrol. Our laboratory previously screened a strain of Aspergillus niger that has a strong degradation effect from brick tea.

Method used

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  • Aspergillus oryzae capable of effectively degrading diethylstilbestrol
  • Aspergillus oryzae capable of effectively degrading diethylstilbestrol
  • Aspergillus oryzae capable of effectively degrading diethylstilbestrol

Examples

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example 1

[0017] Example 1: Degradation of diethylstilbestrol by strain M-4 at different culture times.

[0018] Seed solution preparation: pick M-4 strains and streak on the PDA plate, culture in a 28°C constant temperature incubator for 72 hours, pick a single colony and streak on the PDA slant medium, and culture in a 28°C constant temperature incubator 72h; the M-4 bacterial lawn on the slant culture medium was eluted with sterile physiological saline and mixed evenly to make seed liquid (spore concentration was about 10 8 CFU / mL).

[0019] Take 1mL of seed solution and inoculate it in 29mL (250mL Erlenmeyer flask) of MM medium containing 0.2% Tween 80 with a diethylstilbestrol concentration of 100mg / L, and replace the seed solution with 1mL of normal saline as the blank control. Shake culture at 180r / min for 9 days, take samples every 24 hours, ultrasonically extract with methanol, and detect the residual concentration of diethylstilbestrol by HPLC. The HPLC spectra of diethylsti...

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Abstract

The invention relates to the field of environmental management by virtue of microorganisms and in particular relates to aspergillus oryzae capable of metabolising diethylstilbestrol. The aspergillus oryzae capable of metabolising diethylstilbestrol has the strain number of M 4 and is registered and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.11645 and the preservation date of November 11th, 2015. The aspergillus oryzae provided by the invention can effectively degrade 100 mg / L diethylstilbestrol and can be used for developing a corresponding environment-friendly biological agent, so that an important resource is provided for degrading or eliminating residual diethylstilbestrol in the environment and food, and the aspergillus oryzae has good application prospect.

Description

technical field [0001] The invention relates to the field of using microorganisms for environmental restoration or reduction of food pollutants, in particular to a strain of Aspergillus oryzae capable of degrading diethylstilbestrol. Background technique [0002] Diethylstilbestrol (DES for short) is a synthetic estrogen drug, which is the most effective among artificial estrogens. Because it can promote animal growth, increase protein deposition and reduce fat, it is used as a growth-promoting agent. It is used as an animal feed additive and is widely used in animal husbandry. [0003] A large number of animal experiments in the 1970s proved that taking DES during pregnancy would increase the risk of reproductive tract cancers in their offspring, such as clear cell adenocarcinoma of the vagina and cervix, vaginal cancer, endometrial cancer, and testicular abnormalities. Since it was discovered that diethylstilbestrol has teratogenic and carcinogenic toxicity, the use of DE...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02A23L5/20C12R1/69A62D101/28
CPCA62D3/02A62D2101/28C12N1/145C12R2001/69
Inventor 刘书亮祝元婷文涛胡凯弟陈数平柴先杜陈姝娟敖晓琳
Owner SICHUAN AGRI UNIV
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