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Aspergillus oryzae capable of effectively degrading 3-PBA (3-phenoxybenzoic acid)

A technology of phenoxybenzoic acid and Aspergillus oryzae, applied in the direction of microorganism-based methods, fungi, microorganisms, etc., can solve problems such as restricting the sustainable development of modern agriculture, blocking biodegradation pathways, and hindering biomineralization. To achieve the effect of safe bacteria source, good bacteria resources and low cost

Inactive Publication Date: 2016-07-13
SICHUAN AGRI UNIV
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Problems solved by technology

While 3-PBA causes secondary pollution of agricultural products, it also hinders the biomineralization of pyrethroid pesticides, thereby blocking the biodegradation pathway of such pesticides completely transformed into non-toxic small molecules, indirectly making agricultural products or The problem of pesticide residues in food is more serious, seriously restricting the sustainable development of modern agriculture in my country
In recent years, the 3-PBA degrading bacteria that have been isolated at home and abroad mainly come from soil and sludge, and the species are mainly Pseudomonas, and there are relatively few reports on fungi that can degrade 3-PBA

Method used

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  • Aspergillus oryzae capable of effectively degrading 3-PBA (3-phenoxybenzoic acid)
  • Aspergillus oryzae capable of effectively degrading 3-PBA (3-phenoxybenzoic acid)
  • Aspergillus oryzae capable of effectively degrading 3-PBA (3-phenoxybenzoic acid)

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example 1

[0016] Example 1: Degradation of 3-PBA by strain M-4 at different culture times.

[0017] Seed solution preparation: pick M-4 strains and streak on the PDA plate, culture in a 30°C constant temperature incubator for 72 hours, pick a single colony and streak on the PDA slant medium, and culture in a 30°C constant temperature incubator 72h; the M-4 bacterial lawn on the slant culture medium was eluted with sterile physiological saline and mixed evenly to make seed liquid (spore concentration was about 10 8 CFU / mL).

[0018] Take 1mL of seed solution and inoculate it into 29mL (250mL Erlenmeyer flask) of PD medium with 3-PBA concentration of 100mg / L, replace the seed solution with 1mL of normal saline as blank control, culture with shaking at 30°C and 180r / min for 5 days, every 24h Sampling, methanol ultrasonic extraction, HPLC detection of 3-PBA residual concentration. The HPLC spectra of 3-PBA before and after the treatment of degrading strain M-4 are shown in figure 1 ; The...

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Abstract

The invention relates to the field of environmental modification or food contaminant reduction by adopting microorganisms, in particular to Aspergillus oryzae capable of effectively degrading 3-PBA (3-phenoxybenzoic acid). The Aspergillus oryzae capable of metabolizing 3-PBA is registered and preserved in the CGMCC (China General Microbiological Culture Collection Center) with the strain No. being M-4, the preservation number being CGMCC No. 11645 and the preservation date being November 11th, 2015. The Aspergillus oryzae can effectively degrade 3-PBA, and the strain can be used for developing corresponding environment-friendly biological agents and can provide a good strain resource for degradation or removal of 3-PBA remaining in the environment or in food such as agricultural and animal products and the like, thereby having a good application prospect.

Description

technical field [0001] The invention relates to the field of using microorganisms for environmental restoration or reduction of food pollutants, in particular to a strain of Aspergillus oryzae capable of degrading 3-phenoxybenzoic acid. Background technique [0002] 3-phenoxybenzoic acid (3-phenoxybenzoic acid, 3-PBA) is one of the main intermediate products in the degradation of most pyrethroid pesticides. 3-PBA is not only a potential environmental pollutant with high toxicity to fungi, but also an estrogen substance with certain reproductive toxicity. It has been reported that 3-PBA exhibits stronger biological toxicity than its parent compound pyrethroids. Compared with pyrethroid pesticides, 3-PBA has a more stable structure and is difficult to degrade under natural conditions, and its half-life is much longer than that of pyrethroid pesticides. Moreover, the mobility of 3-PBA in the soil is stronger, which can cause its expanded pollution in the soil. [0003] In th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/69
CPCC12N1/145C12R2001/69
Inventor 刘书亮祝元婷文涛何利周康韩新锋韩国全
Owner SICHUAN AGRI UNIV
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