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Method for detecting diethylstilbestrol

A technique for diethylstilbestrol and ethanol, applied in the field of detecting diethylstilbestrol, can solve the problems of expensive experimental instruments, high environmental requirements, cumbersome derivatization steps, etc., and achieves the effects of fast detection speed, low detection cost, and simple method and equipment

Inactive Publication Date: 2009-12-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method also has the disadvantages of expensive experimental equipment, complicated maintenance procedures, high environmental requirements for equipment, high technical content, high toxicity of reagents, high cost of single sample detection, and complicated sample pretreatment; gas chromatography-mass spectrometry, radioactivity Immunoassay and gas chromatography with electron capture detector are currently the most effective and sensitive detection methods for the determination of DES ppb level. However, due to the polarity and high boiling point of diethylstilbestrol, tedious derivatization is required chemicalization step, which limits the application of gas chromatography analysis
All of the above analysis methods require large-scale analytical instruments, the operation process is cumbersome, and on-site detection cannot be realized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 5g of minced fresh chicken and put it in a centrifuge tube with stopper, add 10ml of ethanol, heat in an ultrasonic water bath for 30min, centrifuge at 3000rpm for 10min, remove the supernatant, add 10ml of ethanol to the residue, mix well and shake for 20min. Centrifuge at 3000rpm for 10min and combine the two supernatants. At this time, turbidity appears. Centrifuge again for 10min. Take the supernatant through a microfiltration membrane, dilute to 10ml with deionized water, shake well, number separately, and place in a refrigerator at 4℃. In the spare. At about 1.5cm at one end of the paper chromatography strip, use the injection capillary to spot the sample solution, once for each spot, and then add twice to make it into a dot with a diameter of about 5mm. After spotting, the solvent is allowed to evaporate freely. Take 10ml of the n-hexane-ethanol solution in the chromatography cylinder. The n-hexane-ethanol developer is: calculated by volume, 10 parts of n-hexan...

Embodiment 2

[0023] Weigh 5g of minced fresh pork, put it in a centrifuge tube with stopper, add 10ml of ethanol, heat in an ultrasonic water bath for 30min, centrifuge at 3000rpm for 10min, remove the supernatant, add 10ml of ethanol to the residue, mix well and shake for 20min. Centrifuge at 3000rpm for 10min and combine the two supernatants. At this time, turbidity appears. Centrifuge again for 10min. Take the supernatant through a microfiltration membrane, dilute to 10ml with deionized water, shake well, number separately, and place in a refrigerator at 4℃. In the spare. At about 1.5cm at one end of the paper chromatography strip, use the injection capillary to spot the sample solution, once for each spot, and then add twice to make it into a dot with a diameter of about 5mm. After spotting, the solvent is allowed to evaporate freely. Take 10ml of the n-hexane-ethanol solution in the chromatographic cylinder. The n-hexane-ethanol developer is: calculated by volume, 8 parts of n-hexane and ...

Embodiment 3

[0026] Weigh 5g of minced fresh beef, put it in a centrifuge tube with stopper, add 10ml of ethanol, heat in an ultrasonic water bath for 30min, centrifuge at 3000rpm for 10min, remove the supernatant, add 10ml of ethanol to the residue, mix well and shake for 20min. Centrifuge at 3000rpm for 10min and combine the two supernatants. At this time, turbidity appears. Centrifuge again for 10min. Take the supernatant through a microfiltration membrane, dilute to 10ml with deionized water, shake well, number separately, and place in a refrigerator at 4℃. In the spare. At about 1.5cm at one end of the paper chromatography strip, use the injection capillary to spot the sample solution, once for each spot, and then add twice to make it into a dot with a diameter of about 5mm. After spotting, the solvent is allowed to evaporate freely. Take 10ml of n-hexane-ethanol solution in the chromatographic cylinder, calculate by volume, mix 5 parts of n-hexane and 0.1 part of ethanol; put the paper c...

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Abstract

A method for detecting diethylstilbestrol belongs to the field of analytical chemistry, wherein the method includes the followings: pre-treating the sample to be detected, preparing the sample solution and then carrying out the following treatments: adding sample solution at one end point of the paper chromatography strip; placing the paper chromatography strip in the normal hexane-ethanol developing solvent; taking out the paper chromatography strip when the front end of the developing solvent is adjacent to the front end of the paper chromatography strip; air-drying, wherein the normal hexane-ethanol developing solvent by volume includes: 1 to 20 parts of normal hexane, 0.1 to 5 parts of ethanol; spraying the potassium ferricyanide-ferric trichloride-H2O2 on the chromatography strip; color displaying, wherein the potassium ferricyanide-ferric trichloride-H2O2 is as follows: mixing the 0.01 to 5 mol / L aqueous solution of the potassium ferricyanide and the 0.01 to 5 mol / L aqueous solution of ferric trichloride evenly; then adding 0.001% to 5% of the H2O2 by volume 1 to 1; and the volume of the H2O2 is 0 to 10% of the total volume of the solution. The invention has the advantages of simple method and device, fast detecting speed and low detecting cost, and can be used for fastly detecting the DES in food on the spot.

Description

Technical field [0001] The invention relates to a detection method in the technical field of chemical engineering, in particular to a method for detecting ethylene estrol (DES). Background technique [0002] Diethylstilbestrol (DES) was born in the 1940s. It is a synthetic estrogen with a phenolic hydroxyl structure. It has two isomers, cis and trans, of which the trans has strong physiological activity. Because DES can promote animal weight gain, increase protein deposition and reduce fat deposition, it has been widely used to promote animal growth, increase lean meat rate, and improve feed conversion rate. In the late 1970s, DES was found to have reproductive and genetic toxicity (including carcinogenic and teratogenic effects). Therefore, the United States has banned the use of DES as a growth-promoting agent for food animals in 1979, and the European Union has banned the use of DES for food animals in 1986 and restricted the import of food produced by animals that have used t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 王正武宋启军赵波
Owner SHANGHAI JIAO TONG UNIV
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