A kind of estrogen hapten, complete antigen and preparation method thereof

A complete antigen and hapten technology, which is applied in the field of immunochemistry and detection, can solve the problems of low product purity, long reaction time, and uncontrollable reaction, and achieve high product purity, no need for heat treatment, and the effect of saving reaction substrates
CN107698441BActive Publication Date: 2021-05-04广州安基利生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州安基利生物科技有限公司
Publication Date
2021-05-04

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Abstract

The invention discloses an estrogen hapten, a complete antigen and a preparation method thereof. The method comprises: reacting estrols and monochloroacetate in an organic solvent at normal temperature and in the presence of a catalyst, terminating the reaction with ice water, taking the unreacted estrols of the organic extractant, and using acid Acidification produces a white precipitate, which is the estrol hapten. By optimizing the catalyst and reaction time in the synthesis, this method provides a simple method without heating and controlling the pH, and the hapten can be obtained in a short time; the unreacted product and the substrate are used to remove the unreacted hapten by using the extraction method. At the same time, the obtained estrols can continue to be used through recovery, saving the reaction substrate; finally, the method of crystallization by hydrochloric acid acidification can obtain high-purity estrols haptens; Haptens are linked to carrier proteins to obtain complete antigens.
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Description

technical field

[0001] The invention relates to the technical field of immunochemistry and detection, in particular to an estrol hapten, a complete antigen and a preparation method thereof, in particular to diethylstilbestrol (DES), hexestrol (HEX) and dienestrol (DIS) Hapten preparation method. Background technique

[0002] Environmental estrogen refers to a class of compounds that have estrogenic effects, can simulate natural estrogen, and interfere with endocrine function. Diethylstilbestrol (DES), hexestrol (HEX) and dienestrol (DIS), are non-steroidal anabolic hormones, which can promote the increase of nitrogen retention in animals, and have the effect of increasing protein deposition and reducing fat deposition, because of its simple structure , easy to synthesize, low cost and other characteristics, it is used to promote the growth of livestock and poultry, increase the lean meat rate, etc. Since DES, HEX, DIS, etc. are all environmental estrogens, they can affect ...

Claims

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