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Anti-sildenafil specific antibody

A sildenafil and antibody technology, applied in the biological field, can solve problems such as human harm and major side effects, and achieve the effects of low price, high synthesis efficiency and easy acquisition.

Inactive Publication Date: 2012-02-01
TIANJIN UNIV OF SCI & TECH
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Sildenafil illegally added to functional foods has relatively large side effects and causes great harm to the human body

Method used

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Embodiment 1

[0028] 1. Synthesis of Hapten

[0029] The present invention selects the hapten of sildenafil, and the molecular structure is:

[0030]

[0031] The specific preparation method is: sildenafil (47.5mg, 0.1mmol) is added to 60% sodium hydride (5.2mg, 0.13mmol, dispersed in mineral oil) in N, N-dimethylformamide (1.5mL) In suspension (nitrogen protection). After stirring at room temperature for 1 h, a solution of ethyl bromoacetate (19 mg, 0.11 mmo) in N,N-dimethylformamide (0.8 mL) was added dropwise. After stirring at room temperature for 15 minutes, stirring was continued for 16 hours in an oil bath at 60°C. The N,N-dimethylformamide was divided under reduced pressure, and 1 mL of water was added to the residue, and the product was extracted with dichloromethane. The extracts were combined, washed with water, and the organic layer was separated and dried with anhydrous magnesium sulfate. The desiccant was filtered off, and the filtrate was concentrated under reduced pressure, a...

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Abstract

The invention provides a preparation method of a sildenafil artificial antigen and antibody, and relates to a preparation method of an artificial hapten, antigen and antibody maximizing the retention of a sildenafil structure. A sildenafil hapten prepared by a chemical synthesis method and a carrier KLH protein are connected and synthesized into an artificial antigen; and an antibody is prepared by the following steps: immunizing animals with the artificial antigen, taking blood, separating out antiserum and purifying. By designing and synthesizing the hapten and artificial antigen having a sildenafil structure, the invention highlights the molecule-specific antigenic determinant, overcomes difficulties in chemical synthesis, and produces the high-affinity antibody through animal immunization inducement. Compared with other similar methods, the invention has the characteristics of specificity, sensitivity, accuracy, high speed, convenience, low cost and the like; and the designed and synthesized hapten lays a foundation for the preparation of the antibody having favorable specificity.

Description

Technical field [0001] The present invention relates to selecting a compound containing the original structure of sildenafil as the sildenafil hapten, and making the hapten into an antigen, thereby producing antibodies; and the synthesis of such haptens and antigens and antibody preparation method. The invention belongs to the field of biotechnology. Background technique [0002] With the integration of the global economy and the internationalization of food trade, food safety has become a worldwide challenge and an important global public health issue. With the improvement of people's living standards, people are paying more and more attention to their health, and functional foods are becoming increasingly popular. However, in recent years, the problem of illegally added drugs in functional foods has been very prominent. The illegal addition of sildenafil in functional foods such as "kidney and aphrodisiac" has not only caused great harm to patients, but also brought economic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K16/44C07K16/06C07D487/04G01N33/535
Inventor 王硕张燕王俊平王莉
Owner TIANJIN UNIV OF SCI & TECH
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