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Kit for diagnosing high triglyceride

A technology of hypertriglyceridemia and kits, which is applied in the direction of material analysis, measuring devices, instruments, etc. by observing the influence of chemical indicators, can solve the problems such as unclear mechanism of ApoA5 regulating triglycerides, and achieve Detect the effect of strong specificity, high sensitivity and specificity

Inactive Publication Date: 2006-09-20
廖伟
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AI Technical Summary

Problems solved by technology

However, the mechanism by which ApoA5 regulates triglycerides is unclear

Method used

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  • Kit for diagnosing high triglyceride
  • Kit for diagnosing high triglyceride
  • Kit for diagnosing high triglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The production method of hypertriglyceridemia (FHTG) diagnostic kit:

[0024] 1. Preparation of ApoA5 monoclonal antibody pairs

[0025]Use purified ApoA5 as antigen to immunize BALB / c mice respectively, immunize for the second time at an interval of 21 days, and then immunize once at an interval of 10 days, for a total of 3-4 times, then take out the spleen cells of the immunized mice, and use PEG to The spleen cells were fused with the mouse myeloma cell line SP2 / 0, the hybridoma cells were screened on a 96-well cell culture plate using HAT selective medium, and the anti-ApoA5 cell line was identified by ELISA, and cloned three times to obtain For cell lines that stably secrete highly specific monoclonal antibodies, use the ELISA inhibition method on the same antigen-coated plate, that is, add cell culture supernatant to the reference group, add cell culture supernatant and antigen mixture to the inhibition group, and compare the OD values ​​of the two groups Differe...

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Abstract

This invention relates to kit used to diagnosis hypertriglyceridemia (FHTG). Apolipoprotein A5 (ApoA5) is used as FHTG diagnosis biochemical criterion to make anti ApoA5 single clone antibody amphi. The kit includes enzyme standard anti ApoA5 single clone antibody and unmarked anti ApoA5 single clone antibody, confinig liquid, enzyme substrate solution, peridium buffer solution, termination solution, washing solution and enzyme scale. ApoA5in human body blood is coated by the single antibody, and enzyme anti ApoA5 single clone body is used to recognize ApoA5in human body blood, and they are used to the diagnosis of hypertriglyceridemia to reach efficiency prevention and cure of coronary heart disease and stroke and other cardiovascular disease.

Description

technical field [0001] The invention relates to apolipoprotein A5 (ApoA5) as a biochemical index for the diagnosis of hypertriglyceridemia (FHTG), the preparation technology of anti-ApoA5 antibody pairs, the technology of enzyme-labeled chromogenic immune reaction and the use of ApoA5 antibodies to diagnose high triglycerides The technical field of hypertriglyceridemia (FHTG), in particular relates to a kit and a production method for diagnosing hypertriglyceridemia (FHTG). Background technique [0002] Abnormal apolipoproteins are closely related to coronary heart disease and stroke. Correct diagnosis as early as possible is conducive to the early prevention and treatment of these serious diseases. The development of apolipoprotein diagnostic kit is helpful for the effective prevention and treatment of coronary heart disease and stroke. ApoA5 belongs to the ApoAI / CIII / AIV gene group, is expressed in the liver, and is related to HDL / VLDL. Plasma ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/78G01N33/532
Inventor 廖伟
Owner 廖伟
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