Enzyme-linked immunoassay reagent kit for detecting aquatic animal spiroplasma

Abstract

The invention relates to an enzyme-linked immunity reagent kit which detects an aquatic animal spiroplasma; the reagent kit comprises peridium solution, cleaning solution, calf serum, one-antiserum, enzyme binder working solution, visualization solution, stop solution, positive control, negative control and an enzyme-labeled plate; the visualization solutions are tetramethy benzidine, citric acid-phosphate buffer solution and hydrogen peroxide; the positive control is a sample comprising spiroplasma; the negative control is the sample which doesn't comprise spiroplasma; the one-antiserum is polyclonal antibody of the aquatic animal spiroplasma; the enzyme binder working solution is a horseradish peroxide enzyme-labeled goat anti-rabbit IgG polyclonal antibody; indirect ELISA method is mainly adopted to detect the spiroplasmas in the blood sample and muscle tissue sample of an aquatic animal. The reagent kit has the advantages of convenience, swiftness, economy, high sensitivity, strong specificity and sound repeatability; furthermore, the reagent kit is in favor of popularization and application and can effectively detect the spiroplasmas in blood sample and muscle tissue sample.

Description

technical field [0001] The invention belongs to the biological technology field of aquatic science, and particularly relates to an enzyme-linked immune kit for detecting aquatic animal spiroplasma. Background technique [0002] Spiroplasma microorganisms can spread among freshwater crustaceans and other economic aquatic animals (Crayfish and Penaeus vannamei, etc.), posing a great threat to aquaculture. At present, the detection of aquatic animal spiroplasma pathogens can use light microscopy, electron microscopy, microbiology, and molecular biology methods. These technologies have been used in the routine detection of shrimp and crab spiroplasma pathogens in our laboratory. However, these detection experiments operate It is complex, requires specific professional skills and equipment, and is difficult to popularize and apply in actual production. The ELISA (Enzyme-Linked immunosorbent assay, ELISA) detection method based on the principle of serological reaction can directl...

AI Technical Summary

Problems solved by technology

At present, the detection of spiroplasma pathogens in aquatic animals can use light microscopy, electron microscopy, microbiology, and molecular biology methods. These techniques have been applied to the routine detection of spiroplasma pathogens in shrimp and crabs in our laboratory. It is complex, requires specific professional skills and equipment, and is difficult to promote and apply in actual production