Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Elisa reagent for detecting chloramphenicol and method thereof

An enzyme-linked immunosorbent reagent, chloramphenicol technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problems of chloramphenicol toxicity, premature gray syndrome, granular leukocyte deficiency and other problems

Inactive Publication Date: 2009-09-09
深圳市绿诗源生物技术有限公司
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chloramphenicol has serious toxic and side effects, which can cause human aplastic anemia, agranulocytosis, gray syndrome of newborns and premature infants, etc., and constitute a huge potential threat to human health.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Elisa reagent for detecting chloramphenicol and method thereof
  • Elisa reagent for detecting chloramphenicol and method thereof
  • Elisa reagent for detecting chloramphenicol and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Embodiment 1 detects the preparation of the ELISA kit component of chloramphenicol

[0151] 1. Antigen Synthesis

[0152] a. Synthesis of Coating Progen

[0153] The chloramphenicol hapten is synthesized by a derivative method, and then the hapten is obtained by coupling the hapten with a bovine gamma globulin carrier protein by an active ester method through a diazotization reaction.

[0154] b. Synthesis of Immunogen

[0155] The hapten of chloramphenicol is obtained by coupling the hapten of chloramphenicol with the carrier protein of keyhole limpet hemocyanin by the active ester method.

[0156] 2. Preparation of Chloramphenicol Mouse Monoclonal Antibody

[0157] a. Animal immunity

[0158] Balb / c mice were used as immunized animals, the chloramphenicol hapten and keyhole limpet hemocyanin conjugate was used as the immunogen, the immunization dose was 300 μg / mouse, and the immunogen was mixed with the same amount of Freund's complete The adjuvant was mixed to m...

Embodiment 2

[0169] Embodiment 2 detects the formation of the ELISA kit of chloramphenicol

[0170] Set up the ELISA kit for detecting chloramphenicol so that it includes the following components:

[0171] (1) enzyme-linked plate coated with chloramphenicol antigen;

[0172] (2) Goat anti-mouse anti-antibody labeled with horseradish peroxidase;

[0173] (3) Chloramphenicol mouse monoclonal antibody;

[0174] (4) 6 bottles of chloramphenicol standard solution, the concentrations are 0 μg / L, 0.025 μg / L, 0.075 μg / L, 0.225 μg / L, 0.675 μg / L, 2.025 μg / L;

[0175] (5) The substrate chromogenic solution is a mixed solution of hydrogen peroxide or carbamide peroxide and o-phenylenediamine (OPD) or tetramethylbenzidine (TMB);

[0176] (6) The stop solution is 2mol / L sulfuric acid buffer;

[0177] (7) The concentrated washing solution is deionized water or triple distilled water;

[0178] (8) Antibody diluent is pH 8.2, 0.05 mol / L, phosphate buffer containing 3% horse serum and 5‰ gelatin.

[0...

Embodiment 3

[0180] Embodiment 3 detects the formation of the ELISA kit of chloramphenicol

[0181] Set up the ELISA kit for detecting chloramphenicol so that it includes the following components:

[0182] (1) Enzyme-linked plate coated with goat anti-rabbit anti-antibody;

[0183] (2) chloramphenicol antigen labeled with alkaline phosphatase;

[0184] (3) Chloramphenicol rabbit polyclonal antibody;

[0185](4) 6 bottles of chloramphenicol standard solution, the concentrations are 0 μg / L, 0.025 μg / L, 0.075 μg / L, 0.225 μg / L, 0.675 μg / L, 2.025 μg / L;

[0186] (5) Substrate chromogenic solution p-nitrophosphate buffer;

[0187] (6) The stop solution is 2mol / L sodium hydroxide buffer solution;

[0188] (7) Concentrated washing liquid deionized water or triple distilled water;

[0189] (8) The concentrated complex solution is deionized water or triple distilled water.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an elisa kit for detecting chloramphenicol, comprising an enzyme yoke plate with an encrusting peridium source, an enzyme-labeling substance, a chloramphenicol specific antibody, a chloramphenicol standard solution, a substrate visualization solution, a stopping solution, a thickening and washing solution and a thickening double solution; an encrusting chloramphenicol antigen is obtained by coupling a chloramphenicol hapten and bovine gamma globulin by an active ester method, and an antibody is a polyclonal antibody or a monoclonal antibody. The invention also provides a method for detecting the chloramphenicol in animal-derived food, comprising the following steps: sample pre-treatment; detection by reagents in the kit; and detection result analysis. The elisa kit and the detection method are simple and convenient to operate, have low cost and are suitable for screening a great amount of samples to detect the chloramphenicol in the animal-derived food.

Description

technical field [0001] The invention relates to an ELISA reagent and method for detecting chloramphenicol in the technical field of ELISA and veterinary drug residue detection and analysis. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is the first antibiotic produced by chemical synthesis, which has a strong inhibitory effect on a variety of pathogenic bacteria. Because of its high efficiency and low price, it has been widely used in the prevention and treatment of various infectious diseases in various types of poultry, livestock, bees and aquatic products. However, chloramphenicol has serious toxic and side effects, which can cause human aplastic anemia, agranulocytosis, and gray syndrome in newborns and premature infants, posing a huge potential threat to human health. Therefore, the problem of chloramphenicol residues has attracted great attention from international organizations and many countries and regions. Developed countries such as the Eu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N33/543G01N33/566G01N33/52G01N33/535
Inventor 聂继斌唐俊杨宏温俊梅齐欣吴青李成杨宗繁
Owner 深圳市绿诗源生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com