Different impedance series immunological micro ball and preparing method, method and device for detecting the same

A technology of immune microspheres and series, applied in the field of biomedical testing, can solve problems such as difficulty in ensuring the accuracy of specimen measurement results, long storage time, and unsatisfactory

Inactive Publication Date: 2005-03-02
王占科
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] We take the ELISA technology as an example to illustrate that the current experimental diagnostic technology based on the antigen-antibody reaction generally has the following deficiencies: 1. One reagent can only measure one item
For example, the reagents for measuring hepatitis B virus surface antigen (HBsAg) can only measure HBsAg, but cannot simultaneously measure hepatitis B virus e antigen (HbeAg)
Hospital laboratories or scientific research technicians can only measure one indicator in each experiment. To detect a certain disease-related substance, a lot of repetitive work (such as two-and-a-half series of hepatitis B) must be carried out, which will cost a lot of manpower. Materials and resources, increasing inspection costs
2. Quality control is more difficult
The quality control department can only conduct random sampling inspections from a batch of ELISA assay plates, which is difficult to ensure the accuracy of the specimen assay results
3. It is diffic...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Determination of antigens of certain substances in the body can directly explain the infection of certain substances in the body. The inventor took the simultaneous determination of three antigenic substances of hepatitis B virus (HBsAg, HBeAg, HbcAg) as an example to illustrate the operation steps of the determination of antigenic substances.

[0092] 1) Mix the conductive substance and the latex raw material according to the weight part of 1:1000, 1:2000, 1:3000 to make latex microspheres with different impedances which can be used for chemical covalent bond labeling.

[0093] 2) The latex microspheres are divided into three types: low impedance n1, medium impedance n2 and high impedance n3, and preset impedances n1, n2 and n3 represent the determination of HBsAg, HBeAg and HbcAg respectively, and input the corresponding information into the impedance variable microspheres Liquid biochip detection device software system. The diameters of the three kinds of latex micr...

Embodiment 2

[0105] Determination of antibodies to certain substances in the body can indirectly indicate infection with certain substances in the body. The contriver is example with measuring Hepatitis A virus (HAV) antibody, Hepatitis B virus (HBV) antibody and Hepatitis C virus (HCV) antibody and Hepatitis D virus (HDV) antibody simultaneously, to illustrate the method of antibody substance determination Steps.

[0106] 1) Mix the conductive material and the latex raw material in parts by weight of 1:100, 1:200, 1:300, and 1:400 to make 4 different impedances (n 1 , n 2 , n 3 and n 4 ) characteristic latex microspheres available for covalently binding antigenic proteins.

[0107] 2) Production of genetic engineering products HAV, HBV, HCV and HDV specific antigen fragments or inactivated viral protein particles.

[0108] 3) Four kinds of impedance latex microspheres, n1, n2, n3, and n4, are preset to respectively represent four kinds of antibodies against HAV, HBV, HCV, and HDV, an...

Embodiment 3

[0118] The detection of specific nucleic acid (DNA or RNA) fragments of a certain microorganism can specifically diagnose the existence of the microorganism infection in the body. Nucleic acid can perform specific hybridization according to the principle of complementary base binding. The inventor takes the simultaneous determination of hepatitis A virus (HAV), hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis D virus (HDV) specific DNA or RNA fragments as an example, to illustrate that DNA or RNA class Operation steps for substance determination.

[0119] 1) Mix the conductive material and the latex raw material in parts by weight of 1:10000, 1:20000, 1:30000, and 1:40000 to make four kinds of aminated latex microspheres with different impedances for nucleic acid (DNA) labeling.

[0120] 2) Preset different impedances (n1, n2, n3 and n4) respectively represent HAV, HBV, HCV and HDV special RNA or DNA fragment determination items, and input the impedance variable...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a hospital checking and diagnostic reagent and the instrument development and application field. The technical scheme includes: (1) preparing serial microglobes of different impedances. 2) making the peridium of the known antigen, antibody and DNA segment on different impedance microglobes, making the surface of different impedance serial microglobes bear light signals, 4) interpreting the mpedance and analysing the quantity of light signal. The advantage is that one determining reagent box can determine multiple kinds of specimen. The infinity of impedance variable of the the impedance variable microglobe liquid biochip etermine the infinity of the detecting items, so as to bring more assay items and the higher sensitivity, besides, different impedance diagnosis microglobes can be preserved for a long time, the technical requirement of the determining device is relatively lower, easier for industrialization and popularization.

Description

technical field [0001] The invention belongs to the technical field of biomedical testing. It specifically relates to immune microspheres of different impedance series, a preparation method thereof, and a detection method and device thereof. technical background [0002] At present, experimental diagnostic techniques based on antigen-antibody reactions include enzyme-linked immunosorbent assay (ELISA) technology, radioimmunoassay, luminescent immunoassay, colloidal gold immunoassay, fluorescent immunoassay, luminescent immunoassay, protein chip technology and biological Sensing technology, etc. Experimental diagnostic technology based on nucleic acid molecular hybridization technology, including Northern blot technology, Southern blot technology and in situ hybridization technology, etc. These techniques are common techniques in medical laboratory work, but all have some disadvantages. [0003] We take the ELISA technology as an example to illustrate that the current exper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01J1/00G01N21/64G01N27/04G01N33/533G01N33/546G01N33/547G01N33/553
Inventor 王占科胡新勇祝仲珍柴长春冯青青杨莉萍李国辉
Owner 王占科
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap