Different impedance series immunological micro ball and preparing method, method and device for detecting the same

Abstract

The invention relates to a hospital checking and diagnostic reagent and the instrument development and application field. The technical scheme includes: (1) preparing serial microglobes of different impedances. 2) making the peridium of the known antigen, antibody and DNA segment on different impedance microglobes, making the surface of different impedance serial microglobes bear light signals, 4) interpreting the mpedance and analysing the quantity of light signal. The advantage is that one determining reagent box can determine multiple kinds of specimen. The infinity of impedance variable of the the impedance variable microglobe liquid biochip etermine the infinity of the detecting items, so as to bring more assay items and the higher sensitivity, besides, different impedance diagnosis microglobes can be preserved for a long time, the technical requirement of the determining device is relatively lower, easier for industrialization and popularization.

Description

technical field [0001] The invention belongs to the technical field of biomedical testing. It specifically relates to immune microspheres of different impedance series, a preparation method thereof, and a detection method and device thereof. technical background [0002] At present, experimental diagnostic techniques based on antigen-antibody reactions include enzyme-linked immunosorbent assay (ELISA) technology, radioimmunoassay, luminescent immunoassay, colloidal gold immunoassay, fluorescent immunoassay, luminescent immunoassay, protein chip technology and biological sensor technology, etc. Experimental diagnostic technology based on nucleic acid molecular hybridization technology, including Northern blot technology, Southern blot technology and in situ hybridization technology. These techniques are common techniques in medical laboratory work, but all have some disadvantages. [0003] We take the ELISA technology as an example to illustrate that the current experiment...

AI Technical Summary

Problems solved by technology

[0003] We take the ELISA technology as an example to illustrate that the current experimental diagnostic technology based on the antigen-antibody reaction generally has the following deficiencies: 1. One reagent can only measure one item

For example, the reagents for measuring hepatitis B virus surface antigen (HBsAg) can only measure HBsAg, but cannot simultaneously measure hepatitis B virus e antigen (HbeAg)

Hospital laboratories or scientific research technicians can only measure one indicator in each experiment. To detect a certain disease-related substance, a lot of repetitive work (such as two-and-a-half series of hepatitis B) must be carried out, which will cost a lot of manpower. Materials and resources, increasing inspection costs

2. Quality control is more difficult

The quality control department can only conduct random sampling inspections from a batch of ELISA assay plates, which is difficult to ensure the accuracy of the specimen assay results

3. It is difficult to quantify accurately, the sensitivity is not high, and the operation is complicated

Therefore, the technology to determine the types of substances is not truly infinite.

[0008] 2. Microspheres of different colors may decolorize due to long storage time

[0009] 3. The color of the microsphere will interfere with the determination of the light signal on the surface of the microsphere

[0010] 4. High requirements for measuring equipment

[0011] 5. There may be errors in the detection equipment's fine interpretation of the color types. It is impossible to detect countless substances to be tested at the same time. According to data reports, at most 100 kinds of relatively stable microspheres of different colors can be obtained, which cannot meet the increasing number of laboratories. Testing and Medical Research Program Requirements

[0012] 6. Developing countries have independently developed and produced this kind of measuring equipment, which is not yet mature in technology