Microorganism polyzyme additive agent for improving pigling growth and development
A technology of microorganisms and additives, applied in the field of agricultural products, can solve the problems of easy disease, poor resistance, low application value, etc., and achieve the effect of improving nutritional value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0036] The first step is to cultivate the strains, use the compound mutagenesis breeding technology to optimize each strain, and carry out the fermentation production performance test:
[0037] (1) Use a UV lamp with a power of 15W to add 3ml of each of the bacterial suspensions of Streptococcus lactis, Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, and Lactobacillus casei to 30cm, respectively, and add starch-free medium. In the bacterial plate, irradiate for 1-3 minutes to culture and count, observe the transparent circle on the starch medium, and select the colony whose diameter of the transparent circle and the colony diameter ratio (HC) > 1.5 are transplanted to the inclined surface of the test tube for cultivation;
[0038] Starch medium: peptone 10g, sodium chloride 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g.
[0039] (2) Place 0.5 g of nitrosoguanidine crystals on a 6 mm starch medium plate with bacterial strains, place t...
Embodiment 2
[0054] The first step, with embodiment 1
[0055] Second step, with embodiment 1
[0056] The third step, liquid high-speed culture of bacteria
[0057] (1), each bacterial classification is taken in the following proportions: Streptococcus lactis 20%, Saccharomyces cerevisiae 23%, Bacillus subtilis 16%, Bifidobacterium 26%, Lactobacillus casei 15%;
[0058] (2), strain liquid high-speed culture, with embodiment 1
[0059] Step 4: Solid Synchronous Culture of Compound Bacteria
[0060] Mix the compound microbial agent with carbon source, nitrogen source, inorganic salt and inducer evenly, put it into a closed tank for fermentation, and ferment at 32°C for 8 days, and ferment simultaneously;
[0061] Composition and proportion of solid synchronous medium: green bran 40%, soybean meal 7%, corn flour 10%, molasses 18%, yeast powder 0.8%, magnesium phosphate 0.3%, enzyme inducer 0.1%, sterile water 23.8%; Said enzyme inducer is made up of xylose and sophorose, and its mass rat...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com