Fenitrothion hapten, artificial antigen, specified antibody and use thereof

Abstract

The invention discloses a O, O-dimethyl-O-[-3-methyl-4-amino phenyl] thionic phosphate (FNH2) as fenitrothion hapten, artificial antigen and specific antibody, which is characterized by the following: reducing nitro in the fenitrothion molecular structure into amino under acid condition with zinc powder; reacting to obtain the hapten molecule in connection with carrier protein; diazotizing to couple hapten and protein to obtain immunogen and peridium; using immune animal with immunogen to make the specific antibody.

Description

Fenitrothion hapten, artificial antigen, specific antibody and use thereof technical field The invention belongs to the field of immunology, and in particular relates to a fenitrothion hapten, an artificial antigen and a specific antibody prepared from the antigen, and also relates to the application of the hapten, the artificial antigen and the specific antibody. Background technique Due to the continuous development of pesticide use technology and the continuous expansion of the scale of use, the environmental impact caused by pesticide residues and the chronic and long-term effects on human health are increasingly concerned and worried, and the restrictions on pesticide residues in daily consumption and foreign trade are also increasing. It is becoming more and more strict, and puts forward new requirements and higher standards for the analysis and detection of objects, types, quantities, scope, indicators and many other aspects. In terms of the analysis and detection o...

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Problems solved by technology

At present, there are no dissertations or works dedicated to the study of hapten molecular design and its immune recognition mechanism

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