Combination detection gene chip kit for three kinds of genitourinary infection pathogen

Abstract

The invention relates to a combined test gene chip reagent kit for three urogenital tract infection pathogens, pertaining to the field of biotechnology. The gene chip consists of 18 sites, i.e. 5 specific and conservative test-target sequences of each pathogen of chlamydia trachomatis, mycoplasma urealytium and gonorrhoeae are taken as testing sites, and 2 positive control sites and 1 negative control site are added; PCR method is adopted to expand testing-target sites and DNA segments of the control sites to act as a probe and fixed on the peridium slide to achieve a test gene chip. The matrix of the chip is 9X9, each checking site is repeated by 4 times, and arranged evenly. The reagent kit is capable to meet the requirement of all reagents during the testing process. The testing method involves the combination of PCR expansion, molecular hybridization and fluorescent labeling. The invention has the advantages that the testing cost is low, pollution to testing samples is removed, the specificity and flexibility of testing are excellent. The practical clinic application proves that the flexibility of the invention is as high as 97 to 99.7 percent, and 99 to 99.7 percent for specificity.

Description

Technical field: [0001] The invention relates to a combined detection gene chip kit for three pathogens of urogenital tract infection, belonging to the field of biotechnology. Background technique: [0002] Among the genitourinary tract infectious pathogens, the infection rates of Chlamydia trachomatis, Ureaplasma urealyticum and Neisseria gonorrhoeae were the highest. According to the statistics of my country's relevant departments, there are about 4 million STD patients every year, and they have increased by 20-30% in the past 10 years. Among them, gonorrhea ranks first, accounting for 40.72% of venereal diseases, non-gonococcal urethritis is the third, accounting for 21.85% of venereal diseases, in non-gonococcal urethritis, Chlamydia trachomatis accounts for 40-50%, Ureaplasma urealyticum accounts for 20-30% , That is to say, the infection of Chlamydia trachomatis, Ureaplasma urealyticum and Neisseria gonorrhoeae accounted for more than half of the total number of infec...

AI Technical Summary

Problems solved by technology

Among these methods, the culture method has a long cycle and low sensitivity and accuracy; the immune method can only be detected after the production of antigens and antibodies, and cannot achieve early detection for patients with a low degree of infection; the PCR method has false positives and false negatives that are difficult to control Fluorescence real-time quantitative PCR also has the inherent defects of simple PCR, and the equipment and related detection reagents are expensive

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