Immunodiagnosis kit for detecting III dengue virus NS1 antigen and application thereof

Abstract

The invention discloses an immunodiagnosis kit for detecting III dengue virus NS1 antigen and the application of the kit to the detection of the III dengue virus NS1 antigen. The immunodiagnosis kit for detecting III dengue virus NS1 antigen provided by the invention comprises a microplate coated by peridium monoclonal antibody 1D14A2A10, monoclonal antibody 5D32A17 marked by biotin, positive control, negative control, concentration wash solution, colored solution and stopping solution. The kit can specifically detect that NS1 protein of the III dengue virus and the other three types of serotype dengue virus NS1 have no cross reaction and combines the different antigen sites of the NS1. The immunodiagnosis kit for detecting III dengue virus NS1 antigen can rapidly detect large amounts of samples at the same time, has the characteristics of high specificity, high sensitivity, and the like and important clinical application value, and can rapidly and correctly perform early typing diagnosis to the patients with the dengue virus infection.

Description

technical field [0001] The invention relates to an ELISA kit for detecting dengue virus NS1 antigen and the application of the kit in early typing diagnosis of dengue fever. Background technique [0002] Dengue virus belongs to the Flaviviridae family and is transmitted by Aedes aegypti and Aedes albopictus. There are four serotypes of dengue virus: type I, type II, type III and type IV (referred to as DV1, DV2, DV3 and DV4), any type of dengue virus infection can cause a series of clinical symptoms, manifested as latent infection, fever, dengue fever, or more severe dengue hemorrhagic fever and dengue shock syndrome. Dengue virus is mainly prevalent in tropical and subtropical regions. Nearly more than 100 countries and 2.5 billion people are under threat. In dengue epidemic areas, 4 types of dengue virus are often seen alternately, which increases the risk of repeated infection of different types. sex. Dengue fever has become a mosquito-borne viral infectious disease tha...

AI Technical Summary

Problems solved by technology

Due to the common epitopes between the 4 dengue virus serotypes and with other flavivirus members, there is cross-reaction when detected by traditional serological methods such as hemagglutination inhibition test, IgM and IgG antibody capture ELISA, etc. , the results are prone to false positives, and serological detection methods cannot be used for early diagnosis

Virus isolation is the gold standard for the diagnosis of dengue virus infection and can be further used for the identification of virus serotypes, but this method is time-consuming and requires high laboratory conditions

RT-PCR is highly sensitive and can identify virus serotypes, but its application is limited due to high technical requirements, sample contamination and high price

Images