Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunodiagnosis kit for detecting III dengue virus NS1 antigen and application thereof

A technology for dengue virus and immunodiagnosis, applied in measurement devices, resistance to vector-borne diseases, instruments, etc., can solve the problems of high technical requirements, easily contaminated samples, high price, high sensitivity, and achieve simple sample processing and detection. The method is simple and easy to implement and requires simple results

Inactive Publication Date: 2009-11-11
SOUTHERN MEDICAL UNIVERSITY
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the common epitopes between the 4 dengue virus serotypes and with other flavivirus members, there is cross-reaction when detected by traditional serological methods such as hemagglutination inhibition test, IgM and IgG antibody capture ELISA, etc. , the results are prone to false positives, and serological detection methods cannot be used for early diagnosis
Virus isolation is the gold standard for the diagnosis of dengue virus infection and can be further used for the identification of virus serotypes, but this method is time-consuming and requires high laboratory conditions
RT-PCR is highly sensitive and can identify virus serotypes, but its application is limited due to high technical requirements, sample contamination and high price

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunodiagnosis kit for detecting III dengue virus NS1 antigen and application thereof
  • Immunodiagnosis kit for detecting III dengue virus NS1 antigen and application thereof
  • Immunodiagnosis kit for detecting III dengue virus NS1 antigen and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The detection principle of the present invention is to use 1D14A2A10 as the original coating on the microporous reaction plate, add the treated sample to be tested, and then add the detection antibody 5D32A17 labeled with biotin and the antibody labeled with horseradish peroxidase or alkali The avidin of active phosphatase can be judged by measuring the absorbance value of the sample after the color development is terminated.

[0021] The present invention is a detection kit based on the double-antibody sandwich ELISA method, wherein the capture antibody 1D14A2A10 used is selected from a group of monoclonal antibodies against NS1 protein of dengue virus type III, and can specifically bind dengue type III The NS1 protein of the virus; the detection antibody 5D32A17 is a monoclonal antibody that has cross-reactivity with other three types of dengue viruses, and the two bind to different epitopes respectively. The kit of the present invention can detect type III dengue vir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses an immunodiagnosis kit for detecting III dengue virus NS1 antigen and the application of the kit to the detection of the III dengue virus NS1 antigen. The immunodiagnosis kit for detecting III dengue virus NS1 antigen provided by the invention comprises a microplate coated by peridium monoclonal antibody 1D14A2A10, monoclonal antibody 5D32A17 marked by biotin, positive control, negative control, concentration wash solution, colored solution and stopping solution. The kit can specifically detect that NS1 protein of the III dengue virus and the other three types of serotype dengue virus NS1 have no cross reaction and combines the different antigen sites of the NS1. The immunodiagnosis kit for detecting III dengue virus NS1 antigen can rapidly detect large amounts of samples at the same time, has the characteristics of high specificity, high sensitivity, and the like and important clinical application value, and can rapidly and correctly perform early typing diagnosis to the patients with the dengue virus infection.

Description

technical field [0001] The invention relates to an ELISA kit for detecting dengue virus NS1 antigen and the application of the kit in early typing diagnosis of dengue fever. Background technique [0002] Dengue virus belongs to the Flaviviridae family and is transmitted by Aedes aegypti and Aedes albopictus. There are four serotypes of dengue virus: type I, type II, type III and type IV (referred to as DV1, DV2, DV3 and DV4), any type of dengue virus infection can cause a series of clinical symptoms, manifested as latent infection, fever, dengue fever, or more severe dengue hemorrhagic fever and dengue shock syndrome. Dengue virus is mainly prevalent in tropical and subtropical regions. Nearly more than 100 countries and 2.5 billion people are under threat. In dengue epidemic areas, 4 types of dengue virus are often seen alternately, which increases the risk of repeated infection of different types. sex. Dengue fever has become a mosquito-borne viral infectious disease tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/577
CPCY02A50/30
Inventor 车小燕潘玉先晋晶
Owner SOUTHERN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com