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Immune chromatography test paper and preparation method capable of meanwhile detecting ToRCH infection

A technology of immunochromatographic test paper and chromatographic membrane, which is applied in biological testing, measuring devices, and analytical materials, etc., can solve the problem of low sensitivity, achieve simple operation methods, intuitive observation results, and overcome the effect of a pathogen antibody

Inactive Publication Date: 2008-04-09
SHANDONG MEDICAL BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is exactly to overcome the problem that its sensitivity is not high when carrying out multiple pathogen detection on a test paper at present, provide a kind of immunochromatographic test paper and preparation method that have advantages such as simple in structure, easy to use, high sensitivity to detect ToRCH infection simultaneously

Method used

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  • Immune chromatography test paper and preparation method capable of meanwhile detecting ToRCH infection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The overall appearance of the chromatography test strip: a 12*25mm chromatographic membrane is attached to a 12*80mm PVC board, and the chromatographic membrane is coated with Toxoplasma gondii antigen, rubella virus antigen, cytomegalovirus antigen, Herpes simplex virus type II antigen and anti-antibody of colloidal gold-labeled antibody form a solid-phase chromatographic membrane 3, on which the detection lines are distributed in a ladder-like staggered manner, and the lower end of the chromatographic membrane is sequentially glued with a colloidal gold conjugate pad 2 , sample pad 1 (glass fiber), the upper end of chromatographic membrane is glued with absorbent pad 4 (cotton pulp board), and there is the identification area corresponding to the specific antigen coated on the chromatographic membrane on absorbent pad 4, as shown in Figure 1 Show.

Embodiment 2

[0030] The overall appearance of the chromatography test strip: a 12*25mm chromatographic membrane is attached to a 12*80mm PVC board, and the chromatographic membrane is coated with Toxoplasma gondii antigen, rubella virus antigen, cytomegalovirus antigen, Herpes simplex virus type II antigen and anti-antibody of colloidal gold-labeled antibody form a solid-phase chromatographic membrane 3, on which the detection lines are distributed in a staggered wave shape, and the lower end of the chromatographic membrane is sequentially glued with a colloidal gold conjugate pad 2 1. The sample pad 1 (glass fiber), the upper end of the chromatographic membrane is glued with an absorbent pad 4 (cotton pulp board), and the corresponding antigen region of the absorbent pad 4 is marked with the specific detection antibody name, as shown in Figure 2.

Embodiment 3

[0032] The overall appearance of the chromatography test strip: a 15*25mm chromatographic membrane is attached to a 15*80mm PVC plate, and the chromatographic membrane is coated with toxoplasma gondii antigen, rubella virus antigen, cytomegalovirus antigen, The anti-antibody of herpes simplex virus type I antigen, herpes simplex virus type II antigen, and colloidal gold-labeled antibody forms a solid-phase chromatographic membrane 3, on which the detection lines are distributed in a ladder-like staggered manner, and the lower end of the chromatographic membrane is glued in sequence. There are colloidal gold conjugate pad 2, sample pad 1 (glass fiber), the upper end of the chromatographic membrane is glued with absorbent pad 4 (cotton pulp board), and the antigen region corresponding to absorbent pad 4 is marked with the specific detection antibody name, as shown in Figure 3 Show.

[0033] The preparation method of test paper of the present invention is:

[0034] (1) Preparati...

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Abstract

The invention provides a GICA test paper and a preparation method for simultaneously detecting the ToRCH infection. The invention comprises a placode, a chromatography membrane is pasted on the placode, at least two PSA detection lines are fixed on the peridium of the chromatography membrane, a colloidal gold combo pad and a sample pad are pasted on the lower end of the chromatography membrane orderly, and an adsorption pad is on the upper end of the chromatography membrane. The detection lines criss-cross the chromatography membrane, the adsorption pad has a tag area corresponding to the specific detection line, the test paper stripe is used for simultaneously detecting whether the people have a ToRCH specificity IgM or IgG infection. The test paper stripe overcomes the defect that single test paper stripe can detect a pathogene at one time, and when a plurality of experiments are done on the same test paper stripe, the phenomenon that the CMPX formed by the former detection lines can affect the following detecting is avoided, and the defect that the sensitivity is limited greatly is avoided. The invention has the advantages that the operation is simple, quick and sensitive, and the special device is not needed, and the observed result is visualized, etc. The utility mode is convenient for the clinical detecting and screening.

Description

Background technique [0001] The invention relates to a chromatographic test paper and a preparation method thereof, in particular to an immunochromatographic test paper for simultaneously detecting ToRCH infection and a preparation method thereof. Background technique [0002] ToRCH is a combination of English names of Toxoplasma gondii (Tox), Rubella Virus (RubV), Cytomegalovirus (CMV) and Herpes simplex Virus (HSV), which can cause congenital Pathogens that cause perinatal malformations due to intrauterine infection and perinatal infection. [0003] ToRCH infection in pregnant women usually has no clinical symptoms and is an invisible infection. The diagnosis of intrauterine infection in the fetus should first determine whether the pregnant woman is infected. ToRCH infection in pregnant women poses a great threat to eugenics and population quality. Therefore, ToRCH screening during pregnancy is an important very necessary. At present, hospitals and family planning servic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/52
Inventor 吴坚美韩金祥朱波崔亚洲
Owner SHANDONG MEDICAL BIO TECH RES CENT
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