Ray irradiation method for improving peridium quality of polystyrene micropore plate

Abstract

The invention discloses a ray irradiation method for improving peridium quality of polystyrene micropore plate, which is realized by the following schemes: aligning a bottle opening of the polystyrene micropore plate to a cobalt source plate, implementing gamma-ray irradiation to the polystyrene micropore plate by the cobalt source plate, controlling the minimum absorption dose of the polystyrene micropore plate to be 4.0KGY and controlling the unevenness of irradiation to be 1.3-1.4, wherein the gamma-ray has an energy of 1.33MeV,. The invention uses the characteristics of higher energy and high penetration of 60Cor ray, and performs irradiation processing to the polystyrene micropore plate using certain irradiation dose and special technique, wherein the irradiated micropore plate is suitable for all types of antigen molecules, can effectively improve absorption of the micropore plate to micromolecule polypeptide and obviously improve the sensitivity and uniformity for detecting enzyme linked immunosorbent (ELISA). The method has the advantages of simple operation, stable quality, lower cost, and the like, thereby having enormous promotion and application value.

Description

technical field [0001] The invention relates to the technical field of irradiation processing, in particular to a ray irradiation method for improving the coating quality of polystyrene microporous plates. Background technique [0002] Enzyme-linked immunoassay (ELISA) is a method of using immunological techniques to determine trace substances in specimens. It is widely used in conventional methods of medical testing, that is, to detect antibodies or antigenic substances in body fluids through antigen-antibody reactions to quickly diagnose whether the human body is Infection with viruses such as AIDS, hepatitis, etc. The diagnostic kit used for ELISA detection is a polystyrene microwell plate coated with protein / polypeptide, so the quality of polystyrene microwell plate coating will directly affect the sensitivity and specificity of ELISA detection. , Uniformity has a significant impact. At present, in most cases, small molecular polypeptides are more difficult to be adsor...

AI Technical Summary

Problems solved by technology

At present, in most cases, small molecular polypeptides are more difficult to be adsorbed (coated) by polystyrene microwell plates than large molecular proteins, even if the polystyrene microwells are treated with UV (ultraviolet) radiation, which is better than chemical methods. plate, its coating effect on small molecule peptides (HIV antigen) is still far from meeting the effect requirements