H7 subtype water flowling flu virus hemagglutin in antibody indirect ELISA kit

A detection kit and influenza virus technology, applied in the biological field, can solve problems such as the confusion of influenza antibody detection methods, and achieve the effects of easy large-scale promotion and application, good antigenicity, and broad market prospects

Inactive Publication Date: 2006-03-08
ZHEJIANG UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, due to the particularity of the species of waterfowl, the detection method of its influenza antibody has always been a problem that perplexes the evaluation of the immune effect of waterfowl vaccines and the diagnosis of waterfowl influenza.

Method used

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  • H7 subtype water flowling flu virus hemagglutin in antibody indirect ELISA kit
  • H7 subtype water flowling flu virus hemagglutin in antibody indirect ELISA kit

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Embodiment Construction

[0028]The technical scheme of the present invention is: an indirect ELISA detection kit for H7 subtype waterfowl influenza virus hemagglutinin antibody, the kit is provided with an antibody detection plate, enzyme conjugate working solution, positive control, negative control, sample diluent, 10 × Concentrated washing solution, color developing solution A, color developing solution B and stop solution. The detection plate of the H7 subtype waterfowl influenza virus hemagglutinin antibody indirect ELISA detection kit is a detachable 96-well microtiter plate coated with AIV / H7HA recombinant protein, and the enzyme conjugate working solution is HRP-labeled rabbit anti-waterfowl IgG polyclonal antibody , the positive control is the H7 subtype waterfowl standard positive serum, and the negative control is the waterfowl standard negative serum. The specific preparation is as follows:

[0029] (1) Preparation of AIV / H7 recombinant HA protein

[0030] Use RT-PCR technology to amplif...

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Abstract

This invention discloses indirect ELISA kit for hemagglutinin antibody of H7 subtype water bird flu virus, which contains antibody test board, enzyme conjugate work solution, positive control, negative control, sample diluent, concentrated detergent, color development solution A, color development solution B, and stop solution, wherein the test board is dismountable 96 holes microtiter plate of peridium water bird flu H7 subtype hemagglutinin protein (HA), the enzyme conjugate work solution is HRP rabbit antibody against water bird IgG polyclonal antibody, positive control is H7 subtype water bird flu standard positive serum, negative control is water bird standard negative serum.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a rapid diagnosis method and equipment for a virus antibody of waterfowl, in particular to an indirect ELISA detection kit for H7 subtype waterfowl influenza virus (AIV) hemagglutinin antibody. Background technique [0002] Avian influenza (avian influenza), the full name of avian influenza virus infection, is an infectious disease of poultry caused by type A influenza virus of Orthomyxoviridae and Influenza virus, from respiratory system infection to sepsis, etc., poultry The fatality rate after infection is high. Bird flu was first discovered in Italy by Perroncito in 1878. Its pathogen was discovered for the first time in 1900, and it was not confirmed by serology until 1955 that it belonged to type A (A) influenza virus [1]. Since then, avian influenza viruses have been prevalent in poultry around the world, with varying degrees of impact. In particular, the outbreak of bird flu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/531G01N21/31
Inventor 周继勇陈洪勋申会刚
Owner ZHEJIANG UNIV
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