H7 subtype water flowling flu virus hemagglutin in antibody indirect ELISA kit
A detection kit and influenza virus technology, applied in the biological field, can solve problems such as the confusion of influenza antibody detection methods, and achieve the effects of easy large-scale promotion and application, good antigenicity, and broad market prospects
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[0028]The technical scheme of the present invention is: an indirect ELISA detection kit for H7 subtype waterfowl influenza virus hemagglutinin antibody, the kit is provided with an antibody detection plate, enzyme conjugate working solution, positive control, negative control, sample diluent, 10 × Concentrated washing solution, color developing solution A, color developing solution B and stop solution. The detection plate of the H7 subtype waterfowl influenza virus hemagglutinin antibody indirect ELISA detection kit is a detachable 96-well microtiter plate coated with AIV / H7HA recombinant protein, and the enzyme conjugate working solution is HRP-labeled rabbit anti-waterfowl IgG polyclonal antibody , the positive control is the H7 subtype waterfowl standard positive serum, and the negative control is the waterfowl standard negative serum. The specific preparation is as follows:
[0029] (1) Preparation of AIV / H7 recombinant HA protein
[0030] Use RT-PCR technology to amplif...
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