299 results about "Plate Well" patented technology

A simple, efficient apparatus and method for separation of solid and liquid phases useful in methods of high-throughput combinatorial organic synthesis of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis. In a preferred embodiment, an apparatus and method of removal of liquid phase from solid phase compatible with microtiter plate type array(s) of reaction vessels is disclosed.

The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Because the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

A method and apparatus for effecting rapid thermal cycling of samples, by producing a high-velocity air flow through a closed loop flow path, and energizing an electrical heater within the closed loop flow path to heat the air flowing therethrough to a desired temperature. A sample holder is introduced into the closed loop flow path for exposing the sample holder to the high-velocity heated air flowing therethrough for rapidly heating the sample. The sample is rapidly cooled to a desired temperature by de-energizing the electrical heater, and opening an air outlet from the closed loop flow path, while continuing to produce the high-velocity air flow therethrough.

The present invention relates to a thermal cycler for the carrying out of chemical or biological reactions, such as PCR or other nucleic acid amplification reactions, that is segmented with a plurality of reaction vessel receiving elements. The reaction vessel receiving elements are thermally isolated from each other and provide an airtight seal to prevent liquids or moisture from penetrating below the reaction vessel receiving elements. The reaction vessel receiving elements have several recesses arranged in a pattern to receive the reaction vessels of a single standard microtiter plate and the segmented thermal cycler has a system for independently heating and cooling each of the reaction vessel receiving elements.

Provided are systems for arrays for assaying various biomolecules and other compounds in a microtiter plate format. The wells are designed so as to contain subwells to facilitate fluid communication between subwells and to allow for efficient human or robotic manipulation of samples and assay of binding or other interactions of components within the wells.

This invention discloses novel improvements to conventional microtiter plates, involving integrating microfluidic channels with such microtiter plates to simplify the assay operation, Increase operational speed and reduce reagent consumption. The present invention can be used in place of a conventional microliter plate and can be easily substituted without any changes to the existing instrumentation systems designed for microtiter plates. The invention also discloses a microfluidic device integrated with sample loading wells wherein the entire flow process is capillary driven.

A removable secondary liquid dispensing module for use with an existing, automated liquid handling system that defines a work area having a plurality of discrete work stations. Each of the work stations of the automated liquid handling system includes a lab ware site and alignment structure enabling the removable securing standardized microtiter-plates at respective lab ware site. A plate positioning mechanism is configured to move and position the microtiter plates to and from the lab ware sites of the respective work stations thereof and into engagement with the respective carrier alignment structure thereof. Further, a primary liquid dispensing device is configured for selective contact-type dispensing of discrete quantities of fluid, in the range of about one (1) microliter to about ten (10) milliliters, into the test sites of the microtiter plates secured in the respective alignment structure of the respective work station. The removable secondary liquid dispensing module includes a base member dimensioned to fit substantially within a footprint of a work station, and mounting hardware adapted to removably secure the base member in the work station. A support platform is affixed to the base member, and is configured to support a microtiter-plate. An alignment mechanism is configured to removably receive and secure the a microtiter-plate therein by the plate positioning mechanism of the automated liquid handling system. The support platform and alignment mechanism cooperate to form and provide a lab ware site suitable for secured receipt of a microtiter-plate. The secondary liquid dispensing module further includes a secondary liquid dispensing device which is self-contained and operationally independent from the primary liquid dispensing device. The secondary dispensing device is further adapted for selective non-contact-type dispensing of discrete quantities of fluid, in the range of about one (1) nanoliter to about ten (10) microliters, into the test sites of the microtiter-plate.

The invention relates to a kit for extracting nucleic acid by a magnetic bead-microtiter plate method and a method for extracting nucleic acid by the same. The kit comprises a kit body and a reagent, wherein, the kit body consists of a detachable microtiter plate (2) similar to an Elisa plate as well as a magnetic plate (1), and the reagent comprises magnetic beads, lysis-binding solution, washing solution A, washing solution B and eluent; the detachable microtiter plate (2) consists of micropores (4) and a pore frame (3), the micropores (4) are made from polystyrene, polyethylene or polypropylene, the bottom of each micropore is in a 'U' or 'V' shape, and the micropores (4) are classified into two forms, namely single pores and multi-connected pores; and the magnetic plate (1) is matched with the bottom of the detachable microtiter plate (2) in shape and size. The kit can be used for extracting a large quantity of the nucleic acid from a sample at one time, and has the advantages of low cost and high operating efficiency, and is simple in operation.

Methods and devices are provided for preparing and separating biological analytes. In one embodiment, one or more filtration devices or filter plate wells can be formed by clamping a filter sheet between first and second plates having at least one connecting cavity. First and second moveable core pins positioned on opposed sides of the sheet can be moved to remove portions of the sheet. A molten plastic can be injected to fill the volume between the cores and cavities and to form a liquid-impermeable sleeve wall or walls, each being separately sealed at the bottom to the perimeter of the sheet portion, each having liquid permeable regions of said removed and sealed sheet portion, and each remaining open at the top. Various exemplary configurations for filtration devices or filter plate wells are also disclosed.

The invention relates to a vessel and kit for thermal cycling applications, a method for manufacturing such a vessel. The vessel comprises, in the form of a planar grid having a predefined pitch, a plurality of sample wells each having a well wall, which defines an open well end and a closed well end. According to the invention there are provided a plurality of ribs between pairs of adjacent wells, the ribs being connected to the walls of the wells and extending essentially in a plane perpendicular to the plane of the well grid. The invention enables manufacturing of dense microtiter plates, which are stable enough to be used in high temperature applications and allow for more efficient manufacturing of the plate.

The present invention relates to a three-dimensional bioprinter for printing and / or patterning a single type or multiple types of cells into different geometrical arrangements and other three-dimensional structures, such as tissues. The bioprinter comprises multiple heads that can each be loaded with a different cartridge containing a biomaterial or biological material such as cells in a solution or cells in a hydrogel. Each bioprinter head and cartridge has the ability to heat or cool using Peltier technology. The bioprinter also has the ability to auto calibrate on a bed plate configured to accept a petri dish or microtiter plate.

The present invention relates to a method for detecting the presence of carbapenemase-producing bacteria in a sample, said method comprising the steps of: a) performing cell lysis on a test sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzymatic suspension obtained in step a) with a reagent kit, said reagent kit comprising —a carbapenemase substrate selected from the group consisting of carbapenems and cephamycins, —a pH color indicator which will change color when the pH of the reaction mixture is comprised between 6.4 and 8.4, wherein a color change after step b) indicates the presence of carbapenemase-producing bacteria in the test sample. The invention also relates to a reagent kit, to a microtiter plate and to their uses in detecting the presence of carbapenemase producers in a test sample.

A sample preparation system provides an automated process that dispenses appropriate amounts of solid material into wells of a microtiter plate for mixing into a suspension. The system utilizes an array of hollow tubes that are lowered into a particle reservoir such that particle material is forced up into the tubes. During loading, solid plungers in the tubes are positioned a known distance from the open end of the tubes. When the particle material is to be dispensed, the plungers are lowered to push the particle material out of the tubes and into receiving wells of the microtiter plate. The plunger stroke and internal diameter of the tubes defines the dispensed volume of particle material.

A planar extensible structure in a flat planar flex circuit can be extended out of the plane of the circuit by a distance of more than the width of the structure to carry fluids, electrical signals or optical signals into and out of the circuit. The planar extensible structure may be an Archimedes spiral, a parabolic spiral, a polygonal spiral, a non-spiral extensible shape, or other extensible shape. The invention can be used to siphon fluid samples from each well in a multiple-well microtiter plate into a microfluidic manifold for utility in chemical and biochemical analysis. The invention can also be used to allow electrical interconnect between adjacent circuits or circuit boards, and can allow signals carried in optical fibers to be transferred between optical circuits in different planes.

Ultrasound-assisted contactless stirring of liquids in a resonator cell by microparticles is achieved by repeated creating and destruction of nodal patterns associated with standing waves of various resonance frequencies causing continuous movements of microparticles inside the cell. Swept-frequency sonication methods include using constant or variable rate of frequency change as well as a stepwise change of frequency of the transducer within a predefined range. Other useful steps include initial detection of the set of resonance frequencies and periodic refreshing of that set. Control systems are described including means to automatically detect the resonance frequencies and maintain the operation of the transducer thereon. Advantageous designs of the apparatus are described for use in microstirring, mixing of liquids using magnetic microbeads, microbubbles, microtiter plates, microarray plates, etc.

Microtiter plate made of plastic, having a plurality of wells in a plate which has at least one break line that divides the plate into various segments. The break line includes a joint line which is formed by molding together various segments of the plate.

A backlight module comprises a light guide plate, a circuit board, a plurality of light sources, an outer frame and an optical film. The light guide plate is provided with a light input face and a light output face. The light input face is located on one side of the light guide plate and symmetrically formed by two first inclined planes. The two first inclined planes are mutually connected to form an included angle. The circuit board corresponds to the light input face of the light guide plate, and the plurality of light sources on two second inclined planes are arranged in staggered mode. The plurality of light sources are disposed on the second inclined planes of the circuit board and correspond to the first inclined planes. The outer frame is provided with a plurality of bends so as to form a containing space to match with the light guide plate and contain the circuit board, the light sources and the light guide plate. The optical film is arranged on the light output face of the light guide plate and contained inside the outer frame. The backlight module is capable of reducing weight, enabling emitted light to be more even and also enabling the light sources to correspond to the light guide plate well in position.

A filtration plate includes an upper plate having one or more wells formed therein, a lower plate having one or more wells corresponding to the wells of the upper plate, a filter media separating the upper plate wells from the lower plate wells, and a seal formed at an interface between the upper plate and the lower plate. A method of assembling the filtration plate comprises applying a seal-promoting material to a component of the filtration plate and curing the seal-promoting material to form the seal at an interface between the upper plate and the lower plate.

Peptide libraries containing peptides having the same net charge or same like charge are described, which may be used to screen for substrates of kinases and phosphatases and any other enzyme that causes a difference in net charge in a suitable substrate. The libraries are designed using representative amino acids for one or more classes of amino acids, thereby reducing the number of members in the peptide library. Reducing the number of peptides in the library to a manageable size permits the peptides to be segregated into individual wells of a microtiter plate, where the sequences of suitable substrates are immediately ascertainable upon exposure to enzyme by detecting the position of charge inverted peptide substrates in the plate.

The present invention relates to the field of high throughput analysis of samples. In particular, the present invention is directed to a device, a System and a method for simultaneous tempering of multiple samples. More particular, the invention relates to the simultaneous thermocycling of multiple samples to perform PCR in a microtiter plate format.

A microtiter plate for combinatorial experimentation is disclosed comprising a substrate and multiple microwells, arranged on said substrate in a predetermined pattern and separated from each other by separating zones, each microwell comprising a bottom and an upstanding surface formed by the adjacent separating zones and extending at most 500 mum above the bottom, wherein the composition of said bottoms on the one hand and the composition of said separating zones and upstanding surfaces on the other hand show a different hydrophilicity, and wherein said bottoms have undergone a surface treatment to improve the dynamics of fluid spreading.

An automated dispensing device is provided that dispenses a calibrated quantity of a fluid into a receptacle having a plurality of spaced-apart rows of receiving wells. The calibrated quantity of fluid is determined based upon a dispensing time and a dispensing scale factor that accounts for the viscosity of the fluid to be dispensed. The automated dispensing device can be configured with independently controllable nozzles for selective delivery of fluid. The automated dispensing device is particularly suitable for dispensing a calibrated quantity of ammonium hydroxide into the receiving wells of each row of a microtiter plate for oligonucleotide cleavage.

The present invention relates generally to an apparatus and kit in the microtiter plate format for improved multiplexed microarraying. The apparatus overcomes current limitations with conventional polymeric and glass bottom microtiter plates. The invention also relates to an apparatus for conducting multiple biomolecular assays comprising a low self-fluorescent coated substrate having an upper and lower surface. The substrate upper surface has a pattern comprising a plurality of wells. A flexible superstructure having a plurality of through holes (i.e. openings) in a pattern that is similar to and aligns with the wells on the substrate is removably adherable to the upper surface of the substrate. Preferably, the superstructure is adhesively attachable to the substrate. A tray supports the substrate and the superstructure and acts as an alignment jig. In a preferred embodiment the substrate is low self-fluorescent glass and coated on the upper surface with a functional coating to which other biomolecules can bond.

A precision fluid dispensing system containing a two-piece pump and a precision closed loop controller drive system to address the small volume precision dispensing requirements of bioscience applications. A multiple diameter pump can be combined with a pump having multiple inlet and outlet ports to allow for precision multiple outlet dispenses in a single pump that finds use with microtiter plate pipetting and other precision dispensing. Inlet ports can be located on the smaller diameter of the cylinder with outlet ports on the larger diameter of the cylinder. A dual piston, dual cylinder pump can be used where the upper and lower parts are different diameters and the two pistons are coupled with a fluid layer.

An apparatus accesses and processes reflection images detected from microarray biochips with sidewalls. The apparatus uses a movable system to carry an optical module and a microtiter plate to achieve relative movement in different directions, so that pattern images of all wells in the microtiter plate can be retrieved during a single scanning process. Scanned image data are further compared with pre-defined patterns by a computer program for analysis.

Ultrasound-assisted contactless stirring of liquids in a resonator cell by microparticles is achieved by repeated creating and destruction of nodal patterns associated with standing waves of various resonance frequencies causing continuous movements of microparticles inside the cell. Swept-frequency sonication technique includes using constant or variable rate of frequency change as well as a stepwise change of frequency of the transducer within a predefined range. Other useful provisions include initial detection of the set of resonance frequencies and periodic refreshing of that set. Control systems are described including means to automatically detect the resonance frequencies and maintain the operation of the transducer thereon. Advantageous designs of the apparatus are described for use in microstirring, mixing of liquids using magnetic microbeads, microbubbles, microtiter plates, microarray plates, etc.